X-Git-Url: http://woldlab.caltech.edu/gitweb/?p=erange.git;a=blobdiff_plain;f=cistematic%2Fgenomes%2Fspurpuratus.py;fp=cistematic%2Fgenomes%2Fspurpuratus.py;h=6098562b45b54ea2916987552587e95ab50ab8df;hp=0000000000000000000000000000000000000000;hb=bc30aca13e5ec397c92e67002fbf7a103130b828;hpb=0d3e3112fd04c2e6b44a25cacef1d591658ad181 diff --git a/cistematic/genomes/spurpuratus.py b/cistematic/genomes/spurpuratus.py new file mode 100644 index 0000000..6098562 --- /dev/null +++ b/cistematic/genomes/spurpuratus.py @@ -0,0 +1,108 @@ +########################################################################### +# # +# C O P Y R I G H T N O T I C E # +# Copyright (c) 2003-10 by: # +# * California Institute of Technology # +# # +# All Rights Reserved. # +# # +# Permission is hereby granted, free of charge, to any person # +# obtaining a copy of this software and associated documentation files # +# (the "Software"), to deal in the Software without restriction, # +# including without limitation the rights to use, copy, modify, merge, # +# publish, distribute, sublicense, and/or sell copies of the Software, # +# and to permit persons to whom the Software is furnished to do so, # +# subject to the following conditions: # +# # +# The above copyright notice and this permission notice shall be # +# included in all copies or substantial portions of the Software. # +# # +# THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, # +# EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF # +# MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND # +# NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS # +# BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN # +# ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN # +# CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE # +# SOFTWARE. # +########################################################################### +# +# data for Strongylocentrotus purpuratus +import string +from cistematic.genomes import Genome +from os import environ + +if environ.get("CISTEMATIC_ROOT"): + cisRoot = environ.get("CISTEMATIC_ROOT") +else: + cisRoot = "/proj/genome" + +geneDB = "%s/S_purpuratus/spurpuratus.genedb" % cisRoot + + +def loadChromosome(db, chromPath, chromOutPath): + seqArray = [] + seqLen = 0 + spGenome = Genome("spurpuratus", dbFile=db) + inFile = open(chromPath, "r") + header = inFile.readline() + while header != "": + seqArray = [] + seqLen = 0 + fields = header.split() + if "purpuratus" in header: + idpart = fields[3] + else: + idpart = fields[-1].strip() + + parts = idpart.split("_") + chromID = parts[-1] + currentLine = inFile.readline() + while currentLine != "" and currentLine[0] != ">": + lineSeq = currentLine.strip() + seqLen += len(lineSeq) + seqArray.append(lineSeq) + currentLine = inFile.readline() + + seq = string.join(seqArray, "") + if seqLen < 50000: + print "Added contig %s to database" % chromID + spGenome.addSequence(("spurpuratus", chromID), seq, "chromosome", str(seqLen)) + spGenome.addChromosomeEntry(chromID, chromID, "db") + else: + outFileName = "%s%s.bin" % (chromOutPath, chromID) + outFile = open("%s%s" % (cisRoot, outFileName), "w") + outFile.write(seq) + outFile.close() + print "Added contig file %s to database" % outFileName + spGenome.addChromosomeEntry(chromID, outFileName, "file") + + header = currentLine + + inFile.close() + + +def createDBFile(db): + spGenome = Genome("spurpuratus", version="2.1", dbFile=db) + spGenome.createGeneDB(db) + + +def createDBindices(db): + spGenome = Genome("spurpuratus", version="2.1", dbFile=db) + spGenome.createIndices() + + +def buildSpurpuratusDB(db=geneDB): + chromoPath = "%s/download/Spur2.1_Nmasked.txt" % cisRoot + chromoOutPath = "/S_purpuratus/" + + print "Creating database %s" % db + createDBFile(db) + + print "Loading genomic sequence" + loadChromosome(db, chromoPath, chromoOutPath) + + print "Creating Indices" + createDBindices(db) + + print "Finished creating database %s" % db \ No newline at end of file