X-Git-Url: http://woldlab.caltech.edu/gitweb/?p=erange.git;a=blobdiff_plain;f=geneStartBins.py;h=3c5183730c288f9c61e29f41fb1df69c3e0f992f;hp=cbb3c4a37d9d74cbbb356038c80912a6643200f1;hb=HEAD;hpb=5e4ae21098dba3d1edcf11e7279da0d84c3422e4

diff --git a/geneStartBins.py b/geneStartBins.py
index cbb3c4a..3c51837 100755
--- a/geneStartBins.py
+++ b/geneStartBins.py
@@ -1,21 +1,15 @@
-#
-#  geneStartBins.py
-#  ENRAGE
-#
-
 try:
     import psyco
     psyco.full()
 except:
     pass
 
-# originally from version 1.3 of geneDownstreamBins.py
-from commoncode import *
-from cistematic.genomes import Genome
-from cistematic.core.geneinfo import geneinfoDB
 import sys
+from commoncode import getReadSense, getGeneInfoDict, getHeaderComment
+import pysam
+from cistematic.genomes import Genome
 
-print '%s: version 2.0' % sys.argv[0]
+print "geneStartBins: version 2.1"
 if len(sys.argv) < 4:
     print 'usage: python %s genome rdsfile outfilename [-max regionSize] [-raw] [-cache]' % sys.argv[0]
     print '\n\twhere regionSize is the optional maximum region in bp\n'
@@ -24,7 +18,6 @@ if len(sys.argv) < 4:
 genome = sys.argv[1]
 hitfile =  sys.argv[2]
 outfilename = sys.argv[3]
-
 standardMinDist = 3000
 if '-max' in sys.argv:
     standardMinDist = int(sys.argv[sys.argv.index('-max') + 1])
@@ -42,26 +35,21 @@ if '-cache' in sys.argv:
 
 bins = 10
 standardMinThresh = standardMinDist / bins
-
-hitRDS = readDataset(hitfile, verbose = True, cache=doCache)
-readlen = hitRDS.getReadSize()
+bamfile = pysam.Samfile(hitfile, "rb")
+readlen = getHeaderComment(bamfile.header, "ReadLength")
 normalizationFactor = 1.0
 if normalize:
-    totalCount = len(hitRDS)
+    totalCount = getHeaderComment(bamfile.header, "Total")
     normalizationFactor = totalCount / 1000000.
 
 hg = Genome(genome)
-idb = geneinfoDB(cache=True)
-
 gidDict = {}
-geneinfoDict = idb.getallGeneInfo(genome)
+geneinfoDict = getGeneInfoDict(genome, cache=True)
 featuresDict = hg.getallGeneFeatures()
-
-#infile = open(infilename)
 outfile = open(outfilename,'w')
-
 gidList = hg.allGIDs()
 gidList.sort()
+chromosomeList = [chrom for chrom in bamfile.references if chrom != "chrM"]
 for gid in gidList:
     symbol = 'LOC' + gid
     geneinfo = ''
@@ -72,46 +60,59 @@ for gid in gidList:
         symbol = geneinfo[0][0]
     except:
         print geneinfo
+
     newfeatureList = []
     if len(featureList) == 0:
         continue
+
     for (ftype, chrom, start, stop, fsense) in featureList:
         if (start, stop) not in newfeatureList:
             newfeatureList.append((start, stop))
-    if chrom not in hitDict:
+
+    if chrom not in chromosomeList:
         continue
+
     newfeatureList.sort()
     if len(newfeatureList) < 1:
-        #print '%s %s %d' % (gid, symbol, -1)
-        #outfile.write('%s\t%s\t%d\n' % (gid, symbol, -1))
         continue
+
     glen = standardMinDist / 2
     if fsense == 'F':
         nextGene = hg.leftGeneDistance((genome, gid), glen * 2)
         if nextGene < glen * 2:
-                glen = nextGene / 2
+            glen = nextGene / 2
+
         if glen < 1:
-                glen = 1
+            glen = 1
+
         gstart = newfeatureList[0][0] - glen
         if gstart < 0:
-                gstart = 0
+            gstart = 0
+
         gstop = newfeatureList[0][0]  + glen
     else:
         nextGene = hg.rightGeneDistance((genome, gid), glen * 2)
         if nextGene < glen * 2:
             glen = nextGene / 2
+
         if glen < 1:
             glen = 1
+
         gstart = newfeatureList[-1][1] - glen
         gstop = newfeatureList[-1][1] + glen
+
     tagCount = 0
     if glen < standardMinDist / 2:
         continue
+
     binList = [0] * bins
-    for (tagStart, sense, weight) in hitDict[chrom]:
-        tagStart -= gstart 
+    for alignedread in bamfile.fetch(chrom):
+        tagStart = alignedread.pos - gstart
+        sense = getReadSense(alignedread)
+        weight = 1.0/alignedread.opt('NH')
         if tagStart >= 2 * glen:
             break
+
         if tagStart > 0:
             tagCount += weight
             if fsense == 'R':
@@ -122,13 +123,16 @@ for gid in gidList:
                 rdist = 2 * glen - tagStart
                 binID = rdist / standardMinThresh 
                 binList[binID] += weight
+
     if tagCount < 2:
         continue
+
     print '%s %s %d %d %s' % (gid, symbol, tagCount, glen, str(binList))
     outfile.write('%s\t%s\t%d\t%d' % (gid, symbol, tagCount, glen))
     for binAmount in binList:
         outfile.write('\t%d' % binAmount)
+
     outfile.write('\n')
-#infile.close()
+
 outfile.close()