X-Git-Url: http://woldlab.caltech.edu/gitweb/?p=erange.git;a=blobdiff_plain;f=runSNPAnalysis.py;fp=runSNPAnalysis.py;h=e511d806f197ebb8a83846b39e88b94fc5544674;hp=0000000000000000000000000000000000000000;hb=cfc5602b26323ad2365295145e3f6c622d912eb4;hpb=c4561c55cfa9726530c6777b6515c4ef66306b2f

diff --git a/runSNPAnalysis.py b/runSNPAnalysis.py
new file mode 100644
index 0000000..e511d80
--- /dev/null
+++ b/runSNPAnalysis.py
@@ -0,0 +1,100 @@
+import sys
+import optparse
+from commoncode import writeLog
+from makeSNPtrack import makeSNPtrack
+from getNovelSNPs import getNovelSNPsFromFile
+from getSNPGeneInfo import writeSNPGeneInfo
+from chksnp import chkSNPFile
+from chkSNPrmask import chkSNPrmask
+from getSNPs import writeSNPsToFile
+
+VERSION = "1.0"
+
+def main(argv=None):
+    if not argv:
+        argv = sys.argv
+
+    print "runSNPAnalysis: version %s" % VERSION
+    usage = "usage: python runSNPAnalysis.py genome rdsfile label rmaskdbfile dbsnpfile uniqStartMin totalRatio rpkmfile [cachepages]"
+
+    parser = getParser(usage)
+    (options, args) = parser.parse_args(argv[1:])
+
+    if len(args) < 4:
+        print usage
+        sys.exit(1)
+
+    genome = args[0]
+    rdsfile = args[1]
+    label = args[2]
+    rmaskdbfile = args[3]
+    dbsnpfile = args[4]
+    uniqStartMin = args[5]
+    totalRatio = args[6]
+    rpkmfile = args[7]
+    try:
+        cachepages = args[8]
+    except IndexError:
+        cachepages = None
+
+    runSNPAnalysis(genome, rdsfile, label, rmaskdbfile, dbsnpfile, uniqStartMin, totalRatio, rpkmfile, cachepages)
+
+
+def getParser(usage):
+    parser = optparse.OptionParser(usage=usage)
+
+    return parser
+
+
+def runSNPAnalysis(genome, rdsfile, label, rmaskdbfile, dbsnpfile, uniqStartMin, totalRatio, rpkmfile, cachepages=None):
+    """ based on original script runSNPAnalysis.sh
+        usage: runSNPAnalysis.sh genome rdsfile label rmaskdbfile dbsnpfile uniqStartMin totalRatio rpkmfile [cachepages]
+               where for each position S:
+                   uniqStartMin = # independent reads supporting base change at S
+                   totalRatio = total # reads supporting base change at S / total # reads that pass through S
+    """
+
+    # log the parameters
+    message = "with parameters: %s %s %s %s %s %s %s %s" % (genome, rdsfile, label, rmaskdbfile, dbsnpfile, uniqStartMin, totalRatio, rpkmfile)
+    writeLog("rna.log", "runStandardAnalysis.py", message)
+
+    # get all SNPs by extracting it from the RDS
+    fullsnpfilename = "%s.snps.txt" % label
+    try:
+        snpCache = int(cachepages)
+    except TypeError, ValueError:
+        snpCache = 0
+
+    doCache = cachepages is not None
+    writeSNPsToFile(rdsfile, uniqStartMin, totalRatio, fullsnpfilename, doCache, cachePages=snpCache, forceChr=True)
+
+    # get SNPs in non-repeat regions only
+    snpfilename = "%s.nr_snps.txt" % label
+    chkSNPrmask(rmaskdbfile, fullsnpfilename, snpfilename, repeats=False, cachePages=cachepages)
+
+    # Check to see if SNPs are found in dbSNP
+    # if dbSNP128.db is not built yet, build it by running buildsnpdb.py - build snp database using the dbSNP database file downloaded from UCSC
+    # usage: python2.5 buildsnpdb.py snpdbdir snpdbname
+    # the database flat file must be in the snpdbdir directory
+    # To build dbSNP database file, run the following command
+    # python2.5 buildsnpdb.py snp128.txt dbSNP128
+
+    # get dbSNP info for SNPs that are found in the dbSNP database
+    dbsnpFileName = "%s.nr_dbsnp.txt" % label
+    chkSNPFile(dbsnpfile, snpfilename, dbsnpFileName, cachePages=cachepages)
+
+    # get gene info for the snps found in dbSNP
+    genefilename = "%s.nr_dbsnp_geneinfo.txt" % label
+    writeSNPGeneInfo(genome, dbsnpFileName, rpkmfile, genefilename, doCache=doCache)
+
+    # get gene info for snps that are not found in dbSNP
+    outfilename = "%s.nr_final.txt" % label
+    getNovelSNPsFromFile(genome, genefilename, outfilename)
+
+    # make bed file for displaying the snps on UCSC genome browser
+    bedfilename = "%s.nr_snps.bed" % label
+    makeSNPtrack(snpfilename, label, bedfilename)
+
+
+if __name__ == "__main__":
+    main(sys.argv)
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