# Lane 1 : Tile 1 : Raw : Read 1 4685939 bases of sequence found 55933 were errors 8532 were blanks 1.20 percent error rate 0.18 percent blank rate unique alignments : 126647 (total score 15145668) cycles : 37 Breakdown of errors by cycle Cycle: Err pc: Blank pc: 1 0.87 0.17 2 1.60 0.08 3 1.56 0.06 4 1.38 0.06 5 1.36 0.07 6 1.48 0.06 7 0.98 0.06 8 0.92 0.06 9 1.01 0.06 10 1.03 0.07 11 1.02 0.06 12 0.89 0.06 13 0.88 0.07 14 0.84 0.05 15 0.91 0.08 16 0.87 0.06 17 0.96 0.05 18 0.94 0.06 19 1.04 0.06 20 0.95 0.07 21 0.90 0.07 22 0.86 0.07 23 0.99 0.13 24 0.96 0.08 25 0.96 0.08 26 0.99 0.09 27 1.03 0.18 28 1.08 0.18 29 1.07 0.26 30 1.16 0.33 31 1.28 0.40 32 1.25 0.44 33 1.64 0.54 34 2.03 0.58 35 2.00 0.64 36 2.14 0.67 37 2.45 0.61 Error rate relative to reference base (including blanks) (Given a reference base, what is it sequenced as?) Really: Read As: N pc A pc C pc G pc T pc A 0.18 97.92 1.25 0.39 0.26 C 0.18 0.23 98.95 0.23 0.41 G 0.18 0.29 0.27 98.80 0.47 T 0.19 0.17 0.51 0.23 98.89 Error rate relative to reference base (excluding blanks) (Given a reference base, what is it sequenced as?) Really: Read As: A pc C pc G pc T pc A 98.09 1.25 0.39 0.26 C 0.23 99.13 0.23 0.41 G 0.30 0.27 98.97 0.47 T 0.17 0.51 0.23 99.08 Error rate relative to sequenced base (excluding ref blanks) (Given a sequenced base, what was it really?) Read As: Really: A pc C pc G pc T pc N 26.16 24.03 23.42 26.39 A 99.34 0.22 0.28 0.17 C 1.35 97.86 0.26 0.52 G 0.42 0.23 99.12 0.24 T 0.28 0.40 0.46 98.87 Breakdown of errors by nucleotide Read As: Really: A ct C ct G ct T ct N 2232 2050 1998 2252 A 1212263 2648 3363 2039 C 15505 1122933 3028 6007 G 4789 2561 1127774 2713 T 3268 4651 5361 1158477 Full breakdown of errors by cycle and nucleotide Cycle: Read As: Really: A ct C ct G ct T ct 1 N 71 39 54 50 1 A 43465 56 120 66 1 C 218 26692 40 80 1 G 127 55 30058 33 1 T 87 129 92 25115 2 N 37 14 29 23 2 A 42361 37 101 58 2 C 1165 21533 73 159 2 G 113 36 33301 58 2 T 58 65 100 27326 3 N 16 26 15 16 3 A 33739 44 75 56 3 C 1037 30720 97 230 3 G 104 25 27687 47 3 T 76 98 85 32454 4 N 25 19 20 15 4 A 30345 31 66 53 4 C 894 31174 102 178 4 G 116 29 33502 45 4 T 79 88 67 29799 5 N 28 21 20 22 5 A 33797 35 72 39 5 C 911 28790 77 206 5 G 113 29 31399 55 5 T 66 65 59 30843 6 N 20 20 17 19 6 A 33160 29 83 34 6 C 1079 31888 103 209 6 G 103 29 31820 35 6 T 53 60 59 27827 7 N 19 28 12 23 7 A 32652 45 76 52 7 C 430 30112 52 174 7 G 105 30 30067 56 7 T 68 77 75 32494 8 N 22 18 19 17 8 A 31797 43 86 42 8 C 366 30009 65 159 8 G 119 44 31022 62 8 T 45 76 60 32576 9 N 23 20 18 18 9 A 31177 45 74 39 9 C 468 31812 68 210 9 G 111 27 30382 53 9 T 54 67 61 31920 10 N 23 15 21 26 10 A 33538 45 76 53 10 C 540 31637 69 166 10 G 94 31 30435 37 10 T 42 85 67 29647 11 N 18 24 21 19 11 A 32806 36 69 42 11 C 493 31165 72 179 11 G 101 26 31285 60 11 T 51 82 74 30024 12 N 19 20 24 19 12 A 31974 34 76 36 12 C 394 31650 70 132 12 G 101 31 30855 43 12 T 56 73 76 30964 13 N 21 23 22 19 13 A 32914 43 80 50 13 C 313 30538 55 144 13 G 90 34 30189 41 13 T 63 76 120 31812 14 N 18 21 15 14 14 A 32945 45 69 33 14 C 275 30682 60 137 14 G 109 35 30134 37 14 T 62 100 98 31758 15 N 26 27 29 25 15 A 32344 43 64 57 15 C 329 31087 63 152 15 G 115 29 30187 49 15 T 71 83 95 31772 16 N 18 14 20 19 16 A 32614 52 73 36 16 C 270 30913 66 105 16 G 98 46 30058 39 16 T 68 97 147 31894 17 N 18 18 17 9 17 A 32326 46 72 45 17 C 343 30336 88 174 17 G 91 50 30262 40 17 T 62 88 117 32445 18 N 24 16 13 18 18 A 32009 46 89 46 18 C 309 31234 66 152 18 G 90 44 30280 41 18 T 62 112 130 31866 19 N 24 18 10 21 19 A 31978 31 83 38 19 C 541 30946 69 151 19 G 91 22 30591 45 19 T 57 103 81 31747 20 N 25 20 17 21 20 A 32248 38 71 42 20 C 359 30888 87 134 20 G 103 30 30297 51 20 T 63 105 120 31928 21 N 21 23 23 16 21 A 32187 68 68 39 21 C 222 31121 66 122 21 G 101 57 30279 46 21 T 78 129 142 31839 22 N 23 21 24 18 22 A 32377 53 62 37 22 C 244 31011 64 123 22 G 118 42 30392 49 22 T 78 86 131 31694 23 N 35 41 38 45 23 A 32161 46 73 47 23 C 352 30770 65 158 23 G 91 45 30185 53 23 T 75 118 133 32116 24 N 27 24 27 22 24 A 31942 61 69 35 24 C 235 31056 76 160 24 G 100 67 30020 55 24 T 78 122 163 32308 25 N 25 26 21 35 25 A 32196 59 99 48 25 C 254 30607 69 160 25 G 89 45 30383 62 25 T 72 117 141 32139 26 N 27 34 34 25 26 A 32311 49 71 35 26 C 340 30453 70 150 26 G 113 44 30371 64 26 T 81 110 125 32140 27 N 61 48 64 55 27 A 31843 49 86 56 27 C 294 30725 74 158 27 G 128 55 30388 74 27 T 85 122 122 32160 28 N 72 49 50 59 28 A 31897 71 89 41 28 C 257 30705 79 126 28 G 128 74 30361 81 28 T 96 138 181 32093 29 N 85 83 74 90 29 A 32231 73 63 45 29 C 259 30448 79 121 29 G 138 83 30203 88 29 T 102 142 161 32079 30 N 108 97 95 123 30 A 31826 102 75 47 30 C 223 30650 87 155 30 G 123 103 30269 92 30 T 86 148 218 32020 31 N 140 121 101 145 31 A 31705 113 96 52 31 C 221 30503 94 161 31 G 143 107 30476 107 31 T 117 177 224 31844 32 N 148 137 133 142 32 A 32084 128 76 50 32 C 215 30236 94 164 32 G 150 118 30292 112 32 T 112 184 179 31893 33 N 188 147 160 191 33 A 31353 150 134 86 33 C 272 30280 118 191 33 G 215 120 30432 129 33 T 156 220 281 31821 34 N 189 175 174 198 34 A 31795 188 148 94 34 C 274 30016 127 157 34 G 260 217 30039 138 34 T 195 298 460 31499 35 N 196 199 192 224 35 A 31483 201 179 130 35 C 309 29892 111 202 35 G 217 223 30127 195 35 T 175 273 305 31808 36 N 208 214 210 217 36 A 31091 189 181 140 36 C 396 30610 116 226 36 G 265 230 29924 219 36 T 205 251 268 31481 37 N 184 190 165 234 37 A 31592 224 219 140 37 C 404 30044 197 242 37 G 316 249 29822 222 37 T 234 287 344 31332 Error rate relative to reference by cycle/nucleotide Cycle: Read As: Really: A prp C prp G prp T prp @1 N 0.002 0.001 0.002 0.002 @1 A 0.989 0.002 0.004 0.003 @1 C 0.005 0.990 0.001 0.003 @1 G 0.003 0.002 0.990 0.001 @1 T 0.002 0.005 0.003 0.991 @2 N 0.001 0.001 0.001 0.001 @2 A 0.969 0.002 0.003 0.002 @2 C 0.027 0.993 0.002 0.006 @2 G 0.003 0.002 0.991 0.002 @2 T 0.001 0.003 0.003 0.989 @3 N 0.000 0.001 0.001 0.000 @3 A 0.965 0.001 0.003 0.002 @3 C 0.030 0.994 0.003 0.007 @3 G 0.003 0.001 0.990 0.001 @3 T 0.002 0.003 0.003 0.989 @4 N 0.001 0.001 0.001 0.000 @4 A 0.965 0.001 0.002 0.002 @4 C 0.028 0.995 0.003 0.006 @4 G 0.004 0.001 0.992 0.001 @4 T 0.003 0.003 0.002 0.990 @5 N 0.001 0.001 0.001 0.001 @5 A 0.968 0.001 0.002 0.001 @5 C 0.026 0.995 0.002 0.007 @5 G 0.003 0.001 0.993 0.002 @5 T 0.002 0.002 0.002 0.990 @6 N 0.001 0.001 0.001 0.001 @6 A 0.964 0.001 0.003 0.001 @6 C 0.031 0.996 0.003 0.007 @6 G 0.003 0.001 0.992 0.001 @6 T 0.002 0.002 0.002 0.989 @7 N 0.001 0.001 0.000 0.001 @7 A 0.981 0.001 0.003 0.002 @7 C 0.013 0.994 0.002 0.005 @7 G 0.003 0.001 0.993 0.002 @7 T 0.002 0.003 0.002 0.991 @8 N 0.001 0.001 0.001 0.001 @8 A 0.983 0.001 0.003 0.001 @8 C 0.011 0.994 0.002 0.005 @8 G 0.004 0.001 0.993 0.002 @8 T 0.001 0.003 0.002 0.991 @9 N 0.001 0.001 0.001 0.001 @9 A 0.979 0.001 0.002 0.001 @9 C 0.015 0.995 0.002 0.007 @9 G 0.003 0.001 0.993 0.002 @9 T 0.002 0.002 0.002 0.990 @10 N 0.001 0.000 0.001 0.001 @10 A 0.980 0.001 0.002 0.002 @10 C 0.016 0.994 0.002 0.006 @10 G 0.003 0.001 0.992 0.001 @10 T 0.001 0.003 0.002 0.991 @11 N 0.001 0.001 0.001 0.001 @11 A 0.980 0.001 0.002 0.001 @11 C 0.015 0.995 0.002 0.006 @11 G 0.003 0.001 0.993 0.002 @11 T 0.002 0.003 0.002 0.990 @12 N 0.001 0.001 0.001 0.001 @12 A 0.982 0.001 0.002 0.001 @12 C 0.012 0.995 0.002 0.004 @12 G 0.003 0.001 0.992 0.001 @12 T 0.002 0.002 0.002 0.993 @13 N 0.001 0.001 0.001 0.001 @13 A 0.985 0.001 0.003 0.002 @13 C 0.009 0.994 0.002 0.004 @13 G 0.003 0.001 0.991 0.001 @13 T 0.002 0.002 0.004 0.992 @14 N 0.001 0.001 0.000 0.000 @14 A 0.986 0.001 0.002 0.001 @14 C 0.008 0.993 0.002 0.004 @14 G 0.003 0.001 0.992 0.001 @14 T 0.002 0.003 0.003 0.993 @15 N 0.001 0.001 0.001 0.001 @15 A 0.984 0.001 0.002 0.002 @15 C 0.010 0.994 0.002 0.005 @15 G 0.003 0.001 0.992 0.002 @15 T 0.002 0.003 0.003 0.991 @16 N 0.001 0.000 0.001 0.001 @16 A 0.986 0.002 0.002 0.001 @16 C 0.008 0.993 0.002 0.003 @16 G 0.003 0.001 0.990 0.001 @16 T 0.002 0.003 0.005 0.994 @17 N 0.001 0.001 0.001 0.000 @17 A 0.984 0.002 0.002 0.001 @17 C 0.010 0.993 0.003 0.005 @17 G 0.003 0.002 0.990 0.001 @17 T 0.002 0.003 0.004 0.992 @18 N 0.001 0.001 0.000 0.001 @18 A 0.985 0.001 0.003 0.001 @18 C 0.010 0.993 0.002 0.005 @18 G 0.003 0.001 0.990 0.001 @18 T 0.002 0.004 0.004 0.992 @19 N 0.001 0.001 0.000 0.001 @19 A 0.978 0.001 0.003 0.001 @19 C 0.017 0.994 0.002 0.005 @19 G 0.003 0.001 0.992 0.001 @19 T 0.002 0.003 0.003 0.992 @20 N 0.001 0.001 0.001 0.001 @20 A 0.983 0.001 0.002 0.001 @20 C 0.011 0.994 0.003 0.004 @20 G 0.003 0.001 0.990 0.002 @20 T 0.002 0.003 0.004 0.992 @21 N 0.001 0.001 0.001 0.000 @21 A 0.987 0.002 0.002 0.001 @21 C 0.007 0.991 0.002 0.004 @21 G 0.003 0.002 0.990 0.001 @21 T 0.002 0.004 0.005 0.993 @22 N 0.001 0.001 0.001 0.001 @22 A 0.986 0.002 0.002 0.001 @22 C 0.007 0.994 0.002 0.004 @22 G 0.004 0.001 0.991 0.002 @22 T 0.002 0.003 0.004 0.993 @23 N 0.001 0.001 0.001 0.001 @23 A 0.983 0.001 0.002 0.001 @23 C 0.011 0.992 0.002 0.005 @23 G 0.003 0.001 0.990 0.002 @23 T 0.002 0.004 0.004 0.991 @24 N 0.001 0.001 0.001 0.001 @24 A 0.986 0.002 0.002 0.001 @24 C 0.007 0.991 0.003 0.005 @24 G 0.003 0.002 0.989 0.002 @24 T 0.002 0.004 0.005 0.992 @25 N 0.001 0.001 0.001 0.001 @25 A 0.987 0.002 0.003 0.001 @25 C 0.008 0.992 0.002 0.005 @25 G 0.003 0.001 0.989 0.002 @25 T 0.002 0.004 0.005 0.991 @26 N 0.001 0.001 0.001 0.001 @26 A 0.983 0.002 0.002 0.001 @26 C 0.010 0.992 0.002 0.005 @26 G 0.003 0.001 0.990 0.002 @26 T 0.002 0.004 0.004 0.992 @27 N 0.002 0.002 0.002 0.002 @27 A 0.982 0.002 0.003 0.002 @27 C 0.009 0.991 0.002 0.005 @27 G 0.004 0.002 0.989 0.002 @27 T 0.003 0.004 0.004 0.989 @28 N 0.002 0.002 0.002 0.002 @28 A 0.983 0.002 0.003 0.001 @28 C 0.008 0.989 0.003 0.004 @28 G 0.004 0.002 0.987 0.002 @28 T 0.003 0.004 0.006 0.991 @29 N 0.003 0.003 0.002 0.003 @29 A 0.982 0.002 0.002 0.001 @29 C 0.008 0.988 0.003 0.004 @29 G 0.004 0.003 0.988 0.003 @29 T 0.003 0.005 0.005 0.989 @30 N 0.003 0.003 0.003 0.004 @30 A 0.983 0.003 0.002 0.001 @30 C 0.007 0.986 0.003 0.005 @30 G 0.004 0.003 0.985 0.003 @30 T 0.003 0.005 0.007 0.987 @31 N 0.004 0.004 0.003 0.004 @31 A 0.981 0.004 0.003 0.002 @31 C 0.007 0.983 0.003 0.005 @31 G 0.004 0.003 0.983 0.003 @31 T 0.004 0.006 0.007 0.986 @32 N 0.005 0.004 0.004 0.004 @32 A 0.981 0.004 0.002 0.002 @32 C 0.007 0.982 0.003 0.005 @32 G 0.005 0.004 0.984 0.003 @32 T 0.003 0.006 0.006 0.986 @33 N 0.006 0.005 0.005 0.006 @33 A 0.974 0.005 0.004 0.003 @33 C 0.008 0.979 0.004 0.006 @33 G 0.007 0.004 0.978 0.004 @33 T 0.005 0.007 0.009 0.982 @34 N 0.006 0.006 0.006 0.006 @34 A 0.972 0.006 0.005 0.003 @34 C 0.008 0.972 0.004 0.005 @34 G 0.008 0.007 0.971 0.004 @34 T 0.006 0.010 0.015 0.982 @35 N 0.006 0.006 0.006 0.007 @35 A 0.972 0.007 0.006 0.004 @35 C 0.010 0.971 0.004 0.006 @35 G 0.007 0.007 0.975 0.006 @35 T 0.005 0.009 0.010 0.977 @36 N 0.006 0.007 0.007 0.007 @36 A 0.967 0.006 0.006 0.004 @36 C 0.012 0.972 0.004 0.007 @36 G 0.008 0.007 0.975 0.007 @36 T 0.006 0.008 0.009 0.975 @37 N 0.006 0.006 0.005 0.007 @37 A 0.965 0.007 0.007 0.004 @37 C 0.012 0.969 0.006 0.008 @37 G 0.010 0.008 0.970 0.007 @37 T 0.007 0.009 0.011 0.974 Information Content By Cycle Bases this cycle Bases so far Cycle: Equiv info: Align: Total: Equiv info: Align: Total: ~1 121135.20 126433 221956 121135.20 126433 221956 ~2 118981.66 126544 220204 240116.88 252977 442160 ~3 118709.06 126574 219216 358825.94 379551 661376 ~4 119387.88 126568 218313 478213.81 506119 879689 ~5 119571.18 126556 217983 597785.00 632675 1097672 ~6 119189.21 126571 217603 716974.19 759246 1315275 ~7 120874.08 126565 217439 837848.25 885811 1532714 ~8 121116.88 126571 217155 958965.12 1012382 1749869 ~9 120779.02 126568 216903 1079744.12 1138950 1966772 ~10 120721.09 126562 217060 1200465.25 1265512 2183832 ~11 120762.78 126565 217584 1321228.00 1392077 2401416 ~12 121319.63 126565 217610 1442547.62 1518642 2619026 ~13 121292.62 126562 217564 1563840.25 1645204 2836590 ~14 121490.08 126579 217562 1685330.38 1771783 3054152 ~15 121105.89 126540 217267 1806436.25 1898323 3271419 ~16 121309.71 126576 216955 1927746.00 2024899 3488374 ~17 120892.80 126585 216878 2048638.75 2151484 3705252 ~18 120964.48 126576 216864 2169603.25 2278060 3922116 ~19 120717.50 126574 216842 2290320.75 2404634 4138958 ~20 120936.81 126564 216644 2411257.50 2531198 4355602 ~21 121079.27 126564 216639 2532336.75 2657762 4572241 ~22 121315.66 126561 216490 2653652.50 2784323 4788731 ~23 120625.85 126488 216289 2774278.25 2910811 5005020 ~24 120734.09 126547 216482 2895012.25 3037358 5221502 ~25 120758.35 126540 216381 3015770.50 3163898 5437883 ~26 120670.38 126527 216482 3136441.00 3290425 5654365 ~27 120291.03 126419 216156 3256732.00 3416844 5870521 ~28 120022.73 126417 216128 3376754.75 3543261 6086649 ~29 119949.20 126315 215933 3496704.00 3669576 6302582 ~30 119421.66 126224 215800 3616125.75 3795800 6518382 ~31 118718.27 126140 215620 3734844.00 3921940 6734002 ~32 118778.77 126087 215509 3853622.75 4048027 6949511 ~33 116782.13 125958 215248 3970405.00 4173985 7164759 ~34 115034.91 125905 215123 4085440.00 4299890 7379882 ~35 114996.52 125830 214997 4200436.50 4425720 7594879 ~36 114382.40 125792 214951 4314819.00 4551512 7809830 ~37 113076.75 125868 215037 4427896.00 4677380 8024867 The 30 most common words with 2 blanks or less: Ranking Occurrences Words 1 411 GTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGT 2 382 TGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTG 3 324 TGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTG 4 271 GTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGT 5 239 GTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGT 6 234 AACACAACACAACACAACACAACACAACACAACACAA 7 214 ACAACACAACACAACACAACACAACACAACACAACAC 8 209 CACAACACAACACAACACAACACAACACAACACAACA 9 206 AAGACAAGACAAGACAAGACAAGACAAGACAAGACAA 10 203 AGACAAGACAAGACAAGACAAGACAAGACAAGACAAG CAAGACAAGACAAGACAAGACAAGACAAGACAAGACA 11 201 CAACACAACACAACACAACACAACACAACACAACACA 12 200 ACACAACACAACACAACACAACACAACACAACACAAC 13 170 ACAAGACAAGACAAGACAAGACAAGACAAGACAAGAC 14 152 TGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTG 15 149 AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG 16 142 AGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG 17 135 CTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCT 18 118 AAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA 19 117 GACAAGACAAGACAAGACAAGACAAGACAAGACAAGA 20 103 TTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTT 21 102 GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA 22 100 GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA 23 80 TTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT TTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTT 24 76 TCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTC 25 53 CTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT 26 47 CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT 27 42 TCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC 28 37 TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTC 29 36 GTCATGTCTTGTCATGTCTTGTCATGTCTTGTCATGT 30 35 CATGTCTTGTCATGTCTTGTCATGTCTTGTCATGTCT The 30 most common blank patterns (N=any nonblank character) Ranking Occurrences Words 1 209206 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2 2010 N.................................... 3 1020 NN................................... 4 727 NNN.................................. 5 472 NNNN................................. 6 441 ..................................... 7 422 NNNNN................................ 8 376 .NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 9 206 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNN....... 10 191 .NNNNNNNNNNNNN.NNNNNNNNNNNNNNNNNNNNNN 11 181 NNNNNNNN..NNNNN...................... 12 180 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN.N 13 162 NNNNNNNNNNNNNNNNNNNNNNNNNNNN......... 14 160 N.NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN...... 15 158 NNNNNNN.NNNNNNNNNNNNNNNNNNNNNNNNNNNNN 16 157 NNNNNNNNNNNNNNNNNNNNNNNNN.NNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNN.NNNNNNNNNN 17 153 NNNNNNNNNNNNNNNNNNNNN.NNNNNNNNNNNNNNN 18 152 NNNNNNNNNNNNNNNNNNNNNN.NNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNN.NNNNNNNN 19 150 NNNN.NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN.... 20 148 NNNNNNNN.NNNNNNNNNNNNNNNNNNNNNNNNNNNN 21 146 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN.NNNNN 22 145 NNNNNNNNNNNNNNNNNNNNNNNNNNNNN.NNNNNNN 23 144 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN.NNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN.NN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN. 24 143 NNNNNNNNNN.NNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNN.NNNNNNNNNNNNNNNNN 25 142 NNN.NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNN.NNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 26 141 NNNNNNNNNNNN.NNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNN.NNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNN.NNNNNN 27 140 NNNNNNNNNNNNNNNNNNNN.NNNNNNNNNNNNNNNN 28 139 NNNNNNNNN.NNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNN.NNNNNNNNNNNN 29 137 NNNNNNNNNNN.NNNNNNNNNNNNNNNNNNNNNNNNN 30 136 NNNNNNNNNNNNNNNNNNNNNNNNNNN.NNNNNNNNN Log likelihood scores Cycle: Read As: Really: A C G T >1 N -30 -65 -47 -51 >1 A 225 -289 -256 -282 >1 C -208 189 -282 -252 >1 G -237 -273 214 -296 >1 T -246 -229 -243 191 >2 N -25 -80 -40 -54 >2 A 233 -306 -262 -286 >2 C -127 118 -249 -215 >2 G -247 -296 220 -276 >2 T -267 -262 -243 208 >3 N -55 -25 -58 -55 >3 A 228 -288 -265 -278 >3 C -147 135 -251 -214 >3 G -242 -304 219 -277 >3 T -263 -252 -258 209 >4 N -33 -49 -46 -63 >4 A 230 -299 -266 -275 >4 C -154 142 -249 -225 >4 G -246 -306 224 -287 >4 T -257 -253 -265 210 >5 N -35 -52 -55 -49 >5 A 236 -298 -267 -293 >5 C -150 138 -258 -216 >5 G -244 -303 220 -275 >5 T -267 -267 -272 221 >6 N -44 -44 -54 -47 >6 A 235 -305 -260 -299 >6 C -147 136 -250 -219 >6 G -249 -304 227 -296 >6 T -272 -266 -267 220 >7 N -52 -28 -76 -40 >7 A 227 -286 -263 -279 >7 C -184 166 -277 -224 >7 G -245 -300 219 -273 >7 T -268 -262 -263 216 >8 N -38 -50 -47 -54 >8 A 226 -287 -256 -288 >8 C -191 170 -267 -228 >8 G -241 -285 213 -270 >8 T -286 -263 -273 225 >9 N -38 -46 -53 -53 >9 A 229 -284 -262 -290 >9 C -183 162 -267 -218 >9 G -243 -305 220 -276 >9 T -277 -267 -272 224 >10 N -43 -66 -48 -35 >10 A 228 -287 -264 -280 >10 C -177 161 -267 -228 >10 G -251 -299 227 -291 >10 T -285 -254 -264 218 >11 N -55 -38 -46 -52 >11 A 234 -296 -267 -289 >11 C -180 162 -264 -224 >11 G -249 -308 222 -271 >11 T -277 -256 -261 216 >12 N -52 -49 -38 -52 >12 A 234 -297 -262 -294 >12 C -190 172 -266 -238 >12 G -248 -299 224 -285 >12 T -274 -262 -261 217 >13 N -48 -43 -45 -54 >13 A 227 -288 -261 -282 >13 C -199 177 -275 -233 >13 G -252 -295 226 -286 >13 T -270 -262 -242 208 >14 N -44 -34 -54 -58 >14 A 235 -286 -267 -300 >14 C -205 181 -271 -235 >14 G -244 -293 222 -291 >14 T -271 -250 -251 208 >15 N -49 -47 -42 -51 >15 A 229 -287 -270 -275 >15 C -197 175 -269 -231 >15 G -242 -301 219 -279 >15 T -265 -258 -252 210 >16 N -46 -60 -40 -43 >16 A 230 -279 -265 -295 >16 C -206 184 -267 -247 >16 G -248 -281 221 -288 >16 T -267 -251 -233 200 >17 N -38 -38 -42 -77 >17 A 229 -284 -265 -285 >17 C -195 170 -254 -224 >17 G -252 -278 222 -288 >17 T -272 -256 -244 208 >18 N -29 -53 -64 -46 >18 A 224 -284 -255 -284 >18 C -200 177 -268 -231 >18 G -252 -283 223 -287 >18 T -271 -245 -239 202 >19 N -30 -48 -79 -39 >19 A 232 -301 -258 -292 >19 C -176 160 -266 -232 >19 G -252 -314 228 -283 >19 T -274 -249 -259 211 >20 N -36 -49 -58 -47 >20 A 232 -293 -265 -288 >20 C -193 172 -255 -236 >20 G -246 -300 221 -277 >20 T -270 -248 -242 204 >21 N -47 -41 -41 -62 >21 A 226 -267 -267 -291 >21 C -214 188 -267 -241 >21 G -247 -272 217 -282 >21 T -261 -239 -235 196 >22 N -43 -49 -41 -57 >22 A 232 -278 -271 -294 >22 C -210 185 -269 -240 >22 G -241 -286 216 -279 >22 T -261 -256 -238 203 >23 N -54 -45 -50 -40 >23 A 228 -284 -264 -283 >23 C -194 172 -268 -229 >23 G -252 -282 220 -275 >23 T -263 -243 -238 199 >24 N -43 -50 -43 -54 >24 A 228 -272 -266 -296 >24 C -212 181 -261 -229 >24 G -247 -265 213 -273 >24 T -262 -242 -229 194 >25 N -51 -49 -61 -31 >25 A 219 -273 -251 -282 >25 C -208 180 -265 -228 >25 G -253 -283 219 -269 >25 T -265 -244 -236 198 >26 N -53 -40 -40 -57 >26 A 231 -282 -265 -296 >26 C -195 173 -264 -231 >26 G -243 -284 213 -267 >26 T -260 -246 -241 200 >27 N -43 -57 -40 -49 >27 A 222 -281 -256 -275 >27 C -202 176 -262 -229 >27 G -237 -274 207 -261 >27 T -258 -242 -242 199 >28 N -34 -56 -55 -46 >28 A 220 -265 -255 -289 >28 C -208 182 -259 -239 >28 G -237 -261 203 -257 >28 T -252 -237 -225 188 >29 N -46 -47 -54 -42 >29 A 225 -264 -271 -285 >29 C -207 182 -259 -240 >29 G -234 -256 199 -253 >29 T -250 -235 -230 189 >30 N -46 -52 -53 -38 >30 A 215 -249 -262 -283 >30 C -214 181 -255 -230 >30 G -239 -247 197 -252 >30 T -257 -233 -217 185 >31 N -41 -50 -60 -39 >31 A 208 -245 -252 -278 >31 C -214 180 -251 -228 >31 G -233 -245 193 -245 >31 T -244 -225 -215 178 >32 N -44 -48 -50 -46 >32 A 210 -240 -262 -281 >32 C -215 180 -251 -227 >32 G -230 -241 190 -243 >32 T -245 -224 -225 182 >33 N -42 -56 -51 -41 >33 A 192 -232 -237 -256 >33 C -205 171 -241 -220 >33 G -215 -240 181 -237 >33 T -231 -216 -205 168 >34 N -46 -50 -50 -43 >34 A 186 -223 -233 -253 >34 C -204 173 -237 -228 >34 G -206 -214 168 -234 >34 T -221 -203 -184 151 >35 N -49 -48 -50 -41 >35 A 179 -219 -224 -238 >35 C -199 168 -243 -217 >35 G -214 -213 167 -219 >35 T -226 -207 -202 162 >36 N -48 -47 -48 -46 >36 A 178 -222 -223 -235 >36 C -189 161 -243 -213 >36 G -205 -212 162 -214 >36 T -219 -210 -207 163 >37 N -50 -48 -56 -36 >37 A 173 -215 -216 -235 >37 C -187 155 -219 -210 >37 G -198 -208 157 -213 >37 T -213 -204 -196 155 Cumulative errors by cycle Cycle: 1 2 3 4 5 !1 125330 126433 126433 126433 126433 !2 123478 126485 126544 126544 126544 !3 121621 126432 126574 126574 126574 !4 119966 126327 126568 126568 126568 !5 118395 126151 126556 126556 126556 !6 116747 125952 126571 126571 126571 !7 115677 125775 126565 126565 126565 !8 114666 125627 126571 126571 126571 !9 113563 125445 126568 126568 126568 !10 112449 125247 126562 126562 126562 !11 111387 125030 126565 126565 126565 !12 110462 124835 126565 126565 126565 !13 109545 124634 126562 126562 126562 !14 108713 124442 126579 126579 126579 !15 107751 124171 126540 126540 126540 !16 106931 123971 126576 126576 126576 !17 106010 123696 126585 126585 126585 !18 105106 123393 126576 126576 126576 !19 104133 123046 126574 126574 126574 !20 103248 122711 126564 126564 126564 !21 102436 122390 126564 126564 126564 !22 101656 122077 126561 126561 126561 !23 100677 121658 126488 126488 126488 !24 99884 121345 126547 126547 126547 !25 99045 120962 126540 126540 126540 !26 98191 120538 126527 126527 126527 !27 97267 119962 126419 126419 126419 !28 96397 119475 126417 126417 126417 !29 95473 118877 126315 126315 126315 !30 94545 118189 126224 126224 126224 !31 93562 117440 126140 126140 126140 !32 92682 116663 126087 126087 126087 !33 91764 115889 125385 125958 125958 !34 90677 115109 124668 125814 125905 !35 89710 114249 123991 125563 125801 !36 88756 113426 123311 125289 125699 !37 87838 112643 122629 125025 125655