From: Rami Rauch <rrauch@stanford.edu>
Date: Mon, 22 Sep 2008 22:33:19 +0000 (+0000)
Subject: converts QuEST peakcalls output file into narrowPeak ENCODE format
X-Git-Url: http://woldlab.caltech.edu/gitweb/?p=htsworkflow.git;a=commitdiff_plain;h=c7cf17f07df74015e0703cf3469470ec86d5a9d6

converts QuEST peakcalls output file into narrowPeak ENCODE format
---

diff --git a/htswanalysis/scripts/QuEST_2_narrowPeak.pm b/htswanalysis/scripts/QuEST_2_narrowPeak.pm
new file mode 100755
index 0000000..3545317
--- /dev/null
+++ b/htswanalysis/scripts/QuEST_2_narrowPeak.pm
@@ -0,0 +1,118 @@
+#!/usr/bin/perl -w
+
+use strict;
+use warnings;
+
+ 
+##~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
+# Transform QuEST peakcalling .out file to naarowPeak format (see doc at http://genomewiki.ucsc.edu/EncodeDCC/index.php/File_Formats)
+# Input rec example: chr8 w: 433 locmax: 5.68905 at: 126511693 nulls: 0.145256 ef: 27.6411204454239
+# w: we don't need.
+# locmax:is the absolute height of the peak. it does not correspond to the actual tag reads, because not all tags contribute equally to the peak determination.
+# at: + 1 is the start position (to adjust for 0 base open, which means start at 1)
+# at: + 2is the end position.
+# nulls: is the control value
+# ef: is the foldof enrichment at that peak, one base
+##~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
+
+# 1. Format into Tab delimited and remove field names inside records
+
+my $track_name = $ARGV[1] || "QuEST Peak Calling";
+
+##############################################################################
+# 2. Find EF Max and nulls MIN
+my $INTFNAME = $ARGV[0].'.INT';
+open (IFILE, "< $ARGV[0]") or die "Can't open file $ARGV[0]";
+open (INTFILE, "> $INTFNAME") or die "Can't open INTERMIDIATE output file";
+
+## vars
+my $i = 0;
+my $delimit = '[\t\s]+';
+my $name = '';
+my $score = 0;
+my $end = 0;
+my $logef = 0;
+my $INTrec = '';
+my $max=-999; # the max ef value
+my $min_ctrl=999; # the minimum background value
+my $seqwindow = 50;
+## print INTFILE "chr\tstart\tend\tname\tef\tlog(ef)\tscore(=rand(log(ef))\n";
+my $r=0; 
+
+while(<IFILE>)
+{
+  chomp;
+  next if($_ =~ /^#/ || $_ =~ /^$/);
+ ## chr8 w: 433 locmax: 5.68905 at: 126511693 nulls: 0.145256 ef: 27.6411204454239
+  $r++;
+  $i++;
+  # print "\nRead record $i: $_";
+  if(!defined($_)) { $i++; print "\nRecord $i not defined."; next; }
+  $INTrec='';
+  my($chr,$x1,$w,$x2,$locmax,$x3,$start,$x4,$nulls,$x5,$ef) = split(/ /,$_);  
+  $start++; # to adjust for 0 base open
+  # apply arbitrary range of 50 bases around the peak
+
+  $end = $start + $seqwindow;
+  $start -= $seqwindow;  
+
+  $name = "peak$i";
+  if($ef eq "NA") {
+    $score="NA";
+  } else {
+    $score = log($ef)/log(2);
+    if($max<$score){$max=$score;}
+    if($min_ctrl>$nulls){$min_ctrl=$nulls;} 
+  }
+  
+  $INTrec = "$chr\t$start\t$end\t$name\t$locmax\t$ef\t$logef\t$score";
+  print INTFILE "$INTrec\n";
+}
+close IFILE;
+close INTFILE;
+print "\n ==== Max EF = $max ===\n";
+
+#################################################
+
+##### Generate 0-1000 score and the narrowPeak score 
+my $NPFNAME = $ARGV[0].'.narrowPeak';
+open (IFILE, "< $INTFNAME") or die "Can't open file $INTFNAME";
+open (NPFILE, "> $NPFNAME") or die "Can't open NARROWPEAK output file $NPFNAME";
+my $b=0;
+my $NPrec = '';
+my $bigscore = 0; # 0-1000 score
+my $graphscore = 0;
+
+while(<IFILE>)
+{
+    $b++;                                                                                                                                                      
+    if(!defined($_)) { $b++; print "\nRecord $b not defined."; next; }
+    chomp;
+    $NPrec = '';
+    my($chr,$start,$end,$name,$locmax,$ef,$logef,$score) = split(/$delimit/,$_);
+    $bigscore = $score;
+    $graphscore = $score;
+    
+    if($graphscore =~ /^NA$/) { $graphscore = log($locmax/$min_ctrl)/log(2);}
+
+    if($graphscore > $max){$max = $graphscore;}
+    $bigscore = int( ($graphscore/$max)*1000 );
+
+    ##$BEDrec = "$chr\t$start\t$end\t$name\t$bigscore";
+    $NPrec = sprintf("%s\t%d\t%d\t%s\t%d\t%s\t%0.3f\t%s\t%d",$chr,$start,$end,$name,$bigscore,'.',$graphscore,'-1',$seqwindow); ## DBG: ,$bigscore,$score,$locmax,$ef);
+    print NPFILE "$NPrec\n";
+}
+
+close NPFILE;
+close IFILE;
+
+print "\n==== F I N I S H E D. Created NARROWPEAK file from QuEST peaks output file, processing $i records ===============\n";
+print "\n ==== Max EF(After clac NA values) = $max ===\n";
+print "\n ==== Min Background = $min_ctrl ===\n";
+print "\n( Temp file is $INTFNAME )";
+print "\n\nOUTPUT file name is $NPFNAME\n";
+
+if($r != $b){ print "\n!!! WARNING !!! SOMETHING'S WRONG: different number of records processed into NARROWPEAK ($b) is different from number of records in INPUT file ($r).\n";}
+
+exit;
+