From: Brandon King Date: Wed, 17 May 2006 16:46:41 +0000 (+0000) Subject: Updated manual X-Git-Url: http://woldlab.caltech.edu/gitweb/?p=mussa.git;a=commitdiff_plain;h=a1ea953cd0a47404ed0a92b70b538662ca12c47b Updated manual * Updated threshold setting based on latest build of Mussagl * Continued outline development * Added FIXME: where ever work needs to be done. --- diff --git a/doc/manual/mussagl_manual.rst b/doc/manual/mussagl_manual.rst index 1a5fb6a..1f348e3 100644 --- a/doc/manual/mussagl_manual.rst +++ b/doc/manual/mussagl_manual.rst @@ -5,9 +5,9 @@ Mussagl Manual By Brandon W. King ------------------ -Last updated: March 23rd, 2006 +Last updated: May 15th, 2006 -Updated to Mussagl build: 141 +Updated to Mussagl build: 141 (Update to 193 in progress) .. contents:: @@ -212,36 +212,67 @@ click open. :align: center +Main Window +----------- + +Overview +~~~~~~~~ +.. Screenshot with numbers showing features. + +Dynamic Threshold +~~~~~~~~~~~~~~~~~ + +Zoom +~~~~ + +Sequence Info +~~~~~~~~~~~~~ + +Scroll Bar +~~~~~~~~~~ +.. Moving sequence + + +Annotations / Motifs +-------------------- + +Annotations +~~~~~~~~~~~ + +Motifs +~~~~~~ + +Load Motifs from File +********************* + +Motif Dialog +************ + + Detailed Info ------------- Threshold ~~~~~~~~~ -The threshold of an analysis is in minimum number of base pair -matches must be meet to in order to be kept as a match. Note that you -can vary the threshold from within Mussagl. For example, if you -choose a `window size`_ of **30** and a **threshold** of **20** the mussa -nway transitive algorithm will store all matches that are 20 out of 30 -bp matches or better and pass it on to Mussagl. Mussagl will -then allow you to dynamically choose a threshold from 10 to 30 base -pairs. A threshold of 30 bps would only show 30 out of 30 bp -matches. A threshold of 20 bps would show all matches of 20 out of 30 -bps or better. Choosing a threshold below 20 in this case won't have -an effect [*]_ because the mussa algorithm didn't report and matches below -this threshold. - -.. [*] In the future, Mussagl will automatically detect the minimum - threshold which was used when defining an analysis and not allow - you to select a threshold below the minimum. See `ticket #52 - `_ for more - info. +The threshold of an analysis is in minimum number of base pair matches +must be meet to in order to be kept as a match. Note that you can vary +the threshold from within Mussagl. For example, if you choose a +`window size`_ of **30** and a **threshold** of **20** the mussa nway +transitive algorithm will store all matches that are 20 out of 30 bp +matches or better and pass it on to Mussagl. Mussagl will then allow +you to dynamically choose a threshold from 20 to 30 base pairs. A +threshold of 30 bps would only show 30 out of 30 bp matches. A +threshold of 20 bps would show all matches of 20 out of 30 bps or +better. If you would like to see results for matches lower than 20 out +of 30, you will need to rerun the analysis with a lower threshold. Window Size ~~~~~~~~~~~ -The typical sizes people tend to choose are between 20 and 30. Feel -free to analysis with this setting depending on your needs. +The typical sizes people tend to choose are between 20 and 30. You +will likely need to experiment with this setting depending on your +needs and input sequence. Sequences @@ -252,6 +283,9 @@ format. Mussa may take a long time to run (>10 minutes) if the total bp length near 280Kb. Once mussa has run once, you can reload previously run analyses. +FIXME: We have learned more about how much sequence and how many to +put in mussagl, this information should be documented here. + Mussa File Formats ------------------ @@ -354,6 +388,9 @@ Motif File Format Format: + +Example: + GGCC 0.0 1 1