X-Git-Url: http://woldlab.caltech.edu/gitweb/?p=pysam.git;a=blobdiff_plain;f=samtools%2Fbam_tview.c;fp=samtools%2Fbam_tview.c;h=0000000000000000000000000000000000000000;hp=7b326fc40e7bf276b7adc3848c0667d5628a68ad;hb=bd0c3067c187d1f718004fb38acc093af8810a02;hpb=1b740fc70684c92a5e2293013217d5a2fd661d8a diff --git a/samtools/bam_tview.c b/samtools/bam_tview.c deleted file mode 100644 index 7b326fc..0000000 --- a/samtools/bam_tview.c +++ /dev/null @@ -1,424 +0,0 @@ -#undef _HAVE_CURSES - -#if _CURSES_LIB == 0 -#elif _CURSES_LIB == 1 -#include -#ifndef NCURSES_VERSION -#warning "_CURSES_LIB=1 but NCURSES_VERSION not defined; tview is NOT compiled" -#else -#define _HAVE_CURSES -#endif -#elif _CURSES_LIB == 2 -#include -#define _HAVE_CURSES -#else -#warning "_CURSES_LIB is not 0, 1 or 2; tview is NOT compiled" -#endif - -#ifdef _HAVE_CURSES -#include -#include -#include -#include "bam.h" -#include "faidx.h" -#include "bam_maqcns.h" - -char bam_aux_getCEi(bam1_t *b, int i); -char bam_aux_getCSi(bam1_t *b, int i); -char bam_aux_getCQi(bam1_t *b, int i); - -#define TV_MIN_ALNROW 2 -#define TV_MAX_GOTO 40 -#define TV_LOW_MAPQ 10 - -#define TV_COLOR_MAPQ 0 -#define TV_COLOR_BASEQ 1 -#define TV_COLOR_NUCL 2 -#define TV_COLOR_COL 3 -#define TV_COLOR_COLQ 4 - -#define TV_BASE_NUCL 0 -#define TV_BASE_COLOR_SPACE 1 - -typedef struct { - int mrow, mcol; - WINDOW *wgoto, *whelp; - - bam_index_t *idx; - bam_lplbuf_t *lplbuf; - bam_header_t *header; - bamFile fp; - int curr_tid, left_pos; - faidx_t *fai; - bam_maqcns_t *bmc; - - int ccol, last_pos, row_shift, base_for, color_for, is_dot, l_ref, ins, no_skip, show_name; - char *ref; -} tview_t; - -int tv_pl_func(uint32_t tid, uint32_t pos, int n, const bam_pileup1_t *pl, void *data) -{ - tview_t *tv = (tview_t*)data; - int i, j, c, rb, attr, max_ins = 0; - uint32_t call = 0; - if (pos < tv->left_pos || tv->ccol > tv->mcol) return 0; // out of screen - // print referece - rb = (tv->ref && pos - tv->left_pos < tv->l_ref)? tv->ref[pos - tv->left_pos] : 'N'; - for (i = tv->last_pos + 1; i < pos; ++i) { - if (i%10 == 0 && tv->mcol - tv->ccol >= 10) mvprintw(0, tv->ccol, "%-d", i+1); - c = tv->ref? tv->ref[i - tv->left_pos] : 'N'; - mvaddch(1, tv->ccol++, c); - } - if (pos%10 == 0 && tv->mcol - tv->ccol >= 10) mvprintw(0, tv->ccol, "%-d", pos+1); - // print consensus - call = bam_maqcns_call(n, pl, tv->bmc); - attr = A_UNDERLINE; - c = ",ACMGRSVTWYHKDBN"[call>>28&0xf]; - i = (call>>8&0xff)/10+1; - if (i > 4) i = 4; - attr |= COLOR_PAIR(i); - if (c == toupper(rb)) c = '.'; - attron(attr); - mvaddch(2, tv->ccol, c); - attroff(attr); - if(tv->ins) { - // calculate maximum insert - for (i = 0; i < n; ++i) { - const bam_pileup1_t *p = pl + i; - if (p->indel > 0 && max_ins < p->indel) max_ins = p->indel; - } - } - // core loop - for (j = 0; j <= max_ins; ++j) { - for (i = 0; i < n; ++i) { - const bam_pileup1_t *p = pl + i; - int row = TV_MIN_ALNROW + p->level - tv->row_shift; - if (j == 0) { - if (!p->is_del) { - if (tv->base_for == TV_BASE_COLOR_SPACE && - (c = bam_aux_getCSi(p->b, p->qpos))) { - c = bam_aux_getCSi(p->b, p->qpos); - // assume that if we found one color, we will be able to get the color error - if (tv->is_dot && '-' == bam_aux_getCEi(p->b, p->qpos)) c = bam1_strand(p->b)? ',' : '.'; - } else { - if (tv->show_name) { - char *name = bam1_qname(p->b); - c = (p->qpos + 1 >= p->b->core.l_qname)? ' ' : name[p->qpos]; - } else { - c = bam_nt16_rev_table[bam1_seqi(bam1_seq(p->b), p->qpos)]; - if (tv->is_dot && toupper(c) == toupper(rb)) c = bam1_strand(p->b)? ',' : '.'; - } - } - } else c = '*'; - } else { // padding - if (j > p->indel) c = '*'; - else { // insertion - if (tv->base_for == TV_BASE_NUCL) { - if (tv->show_name) { - char *name = bam1_qname(p->b); - c = (p->qpos + j + 1 >= p->b->core.l_qname)? ' ' : name[p->qpos + j]; - } else { - c = bam_nt16_rev_table[bam1_seqi(bam1_seq(p->b), p->qpos + j)]; - if (j == 0 && tv->is_dot && toupper(c) == toupper(rb)) c = bam1_strand(p->b)? ',' : '.'; - } - } else { - c = bam_aux_getCSi(p->b, p->qpos + j); - if (tv->is_dot && '-' == bam_aux_getCEi(p->b, p->qpos + j)) c = bam1_strand(p->b)? ',' : '.'; - } - } - } - if (row > TV_MIN_ALNROW && row < tv->mrow) { - int x; - attr = 0; - if (((p->b->core.flag&BAM_FPAIRED) && !(p->b->core.flag&BAM_FPROPER_PAIR)) - || (p->b->core.flag & BAM_FSECONDARY)) attr |= A_UNDERLINE; - if (tv->color_for == TV_COLOR_BASEQ) { - x = bam1_qual(p->b)[p->qpos]/10 + 1; - if (x > 4) x = 4; - attr |= COLOR_PAIR(x); - } else if (tv->color_for == TV_COLOR_MAPQ) { - x = p->b->core.qual/10 + 1; - if (x > 4) x = 4; - attr |= COLOR_PAIR(x); - } else if (tv->color_for == TV_COLOR_NUCL) { - x = bam_nt16_nt4_table[bam1_seqi(bam1_seq(p->b), p->qpos)] + 5; - attr |= COLOR_PAIR(x); - } else if(tv->color_for == TV_COLOR_COL) { - x = 0; - switch(bam_aux_getCSi(p->b, p->qpos)) { - case '0': x = 0; break; - case '1': x = 1; break; - case '2': x = 2; break; - case '3': x = 3; break; - case '4': x = 4; break; - default: x = bam_nt16_nt4_table[bam1_seqi(bam1_seq(p->b), p->qpos)]; break; - } - x+=5; - attr |= COLOR_PAIR(x); - } else if(tv->color_for == TV_COLOR_COLQ) { - x = bam_aux_getCQi(p->b, p->qpos); - if(0 == x) x = bam1_qual(p->b)[p->qpos]; - x = x/10 + 1; - if (x > 4) x = 4; - attr |= COLOR_PAIR(x); - } - attron(attr); - mvaddch(row, tv->ccol, bam1_strand(p->b)? tolower(c) : toupper(c)); - attroff(attr); - } - } - c = j? '*' : rb; - if (c == '*') { - attr = COLOR_PAIR(8); - attron(attr); - mvaddch(1, tv->ccol++, c); - attroff(attr); - } else mvaddch(1, tv->ccol++, c); - } - tv->last_pos = pos; - return 0; -} - -tview_t *tv_init(const char *fn, const char *fn_fa) -{ - tview_t *tv = (tview_t*)calloc(1, sizeof(tview_t)); - tv->is_dot = 1; - tv->idx = bam_index_load(fn); - if (tv->idx == 0) exit(1); - tv->fp = bam_open(fn, "r"); - bgzf_set_cache_size(tv->fp, 8 * 1024 *1024); - assert(tv->fp); - tv->header = bam_header_read(tv->fp); - tv->lplbuf = bam_lplbuf_init(tv_pl_func, tv); - if (fn_fa) tv->fai = fai_load(fn_fa); - tv->bmc = bam_maqcns_init(); - tv->ins = 1; - bam_maqcns_prepare(tv->bmc); - - initscr(); - keypad(stdscr, TRUE); - clear(); - noecho(); - cbreak(); - tv->mrow = 24; tv->mcol = 80; - getmaxyx(stdscr, tv->mrow, tv->mcol); - tv->wgoto = newwin(3, TV_MAX_GOTO + 10, 10, 5); - tv->whelp = newwin(29, 40, 5, 5); - tv->color_for = TV_COLOR_MAPQ; - start_color(); - init_pair(1, COLOR_BLUE, COLOR_BLACK); - init_pair(2, COLOR_GREEN, COLOR_BLACK); - init_pair(3, COLOR_YELLOW, COLOR_BLACK); - init_pair(4, COLOR_WHITE, COLOR_BLACK); - init_pair(5, COLOR_GREEN, COLOR_BLACK); - init_pair(6, COLOR_CYAN, COLOR_BLACK); - init_pair(7, COLOR_YELLOW, COLOR_BLACK); - init_pair(8, COLOR_RED, COLOR_BLACK); - init_pair(9, COLOR_BLUE, COLOR_BLACK); - return tv; -} - -void tv_destroy(tview_t *tv) -{ - delwin(tv->wgoto); delwin(tv->whelp); - endwin(); - - bam_lplbuf_destroy(tv->lplbuf); - bam_maqcns_destroy(tv->bmc); - bam_index_destroy(tv->idx); - if (tv->fai) fai_destroy(tv->fai); - free(tv->ref); - bam_header_destroy(tv->header); - bam_close(tv->fp); - free(tv); -} - -int tv_fetch_func(const bam1_t *b, void *data) -{ - tview_t *tv = (tview_t*)data; - if (tv->no_skip) { - uint32_t *cigar = bam1_cigar(b); // this is cheating... - int i; - for (i = 0; i core.n_cigar; ++i) { - if ((cigar[i]&0xf) == BAM_CREF_SKIP) - cigar[i] = cigar[i]>>4<<4 | BAM_CDEL; - } - } - bam_lplbuf_push(b, tv->lplbuf); - return 0; -} - -int tv_draw_aln(tview_t *tv, int tid, int pos) -{ - // reset - clear(); - tv->curr_tid = tid; tv->left_pos = pos; - tv->last_pos = tv->left_pos - 1; - tv->ccol = 0; - // print ref and consensus - if (tv->fai) { - char *str; - if (tv->ref) free(tv->ref); - str = (char*)calloc(strlen(tv->header->target_name[tv->curr_tid]) + 30, 1); - sprintf(str, "%s:%d-%d", tv->header->target_name[tv->curr_tid], tv->left_pos + 1, tv->left_pos + tv->mcol); - tv->ref = fai_fetch(tv->fai, str, &tv->l_ref); - free(str); - } - // draw aln - bam_lplbuf_reset(tv->lplbuf); - bam_fetch(tv->fp, tv->idx, tv->curr_tid, tv->left_pos, tv->left_pos + tv->mcol, tv, tv_fetch_func); - bam_lplbuf_push(0, tv->lplbuf); - - while (tv->ccol < tv->mcol) { - int pos = tv->last_pos + 1; - if (pos%10 == 0 && tv->mcol - tv->ccol >= 10) mvprintw(0, tv->ccol, "%-d", pos+1); - mvaddch(1, tv->ccol++, (tv->ref && pos < tv->l_ref)? tv->ref[pos - tv->left_pos] : 'N'); - ++tv->last_pos; - } - return 0; -} - -static void tv_win_goto(tview_t *tv, int *tid, int *pos) -{ - char str[256], *p; - int i, l = 0; - wborder(tv->wgoto, '|', '|', '-', '-', '+', '+', '+', '+'); - mvwprintw(tv->wgoto, 1, 2, "Goto: "); - for (;;) { - int c = wgetch(tv->wgoto); - wrefresh(tv->wgoto); - if (c == KEY_BACKSPACE || c == '\010' || c == '\177') { - --l; - } else if (c == KEY_ENTER || c == '\012' || c == '\015') { - int _tid = -1, _beg, _end; - if (str[0] == '=') { - _beg = strtol(str+1, &p, 10); - if (_beg > 0) { - *pos = _beg; - return; - } - } else { - bam_parse_region(tv->header, str, &_tid, &_beg, &_end); - if (_tid >= 0) { - *tid = _tid; *pos = _beg; - return; - } - } - } else if (isgraph(c)) { - if (l < TV_MAX_GOTO) str[l++] = c; - } else if (c == '\027') l = 0; - else if (c == '\033') return; - str[l] = '\0'; - for (i = 0; i < TV_MAX_GOTO; ++i) mvwaddch(tv->wgoto, 1, 8 + i, ' '); - mvwprintw(tv->wgoto, 1, 8, "%s", str); - } -} - -static void tv_win_help(tview_t *tv) { - int r = 1; - WINDOW *win = tv->whelp; - wborder(win, '|', '|', '-', '-', '+', '+', '+', '+'); - mvwprintw(win, r++, 2, " -=- Help -=- "); - r++; - mvwprintw(win, r++, 2, "? This window"); - mvwprintw(win, r++, 2, "Arrows Small scroll movement"); - mvwprintw(win, r++, 2, "h,j,k,l Small scroll movement"); - mvwprintw(win, r++, 2, "H,J,K,L Large scroll movement"); - mvwprintw(win, r++, 2, "ctrl-H Scroll 1k left"); - mvwprintw(win, r++, 2, "ctrl-L Scroll 1k right"); - mvwprintw(win, r++, 2, "space Scroll one screen"); - mvwprintw(win, r++, 2, "backspace Scroll back one screen"); - mvwprintw(win, r++, 2, "g Go to specific location"); - mvwprintw(win, r++, 2, "m Color for mapping qual"); - mvwprintw(win, r++, 2, "n Color for nucleotide"); - mvwprintw(win, r++, 2, "b Color for base quality"); - mvwprintw(win, r++, 2, "c Color for cs color"); - mvwprintw(win, r++, 2, "z Color for cs qual"); - mvwprintw(win, r++, 2, ". Toggle on/off dot view"); - mvwprintw(win, r++, 2, "s Toggle on/off ref skip"); - mvwprintw(win, r++, 2, "r Toggle on/off rd name"); - mvwprintw(win, r++, 2, "N Turn on nt view"); - mvwprintw(win, r++, 2, "C Turn on cs view"); - mvwprintw(win, r++, 2, "i Toggle on/off ins"); - mvwprintw(win, r++, 2, "q Exit"); - r++; - mvwprintw(win, r++, 2, "Underline: Secondary or orphan"); - mvwprintw(win, r++, 2, "Blue: 0-9 Green: 10-19"); - mvwprintw(win, r++, 2, "Yellow: 20-29 White: >=30"); - wrefresh(win); - wgetch(win); -} - -void tv_loop(tview_t *tv) -{ - int tid, pos; - tid = tv->curr_tid; pos = tv->left_pos; - while (1) { - int c = getch(); - switch (c) { - case '?': tv_win_help(tv); break; - case '\033': - case 'q': goto end_loop; - case '/': - case 'g': tv_win_goto(tv, &tid, &pos); break; - case 'm': tv->color_for = TV_COLOR_MAPQ; break; - case 'b': tv->color_for = TV_COLOR_BASEQ; break; - case 'n': tv->color_for = TV_COLOR_NUCL; break; - case 'c': tv->color_for = TV_COLOR_COL; break; - case 'z': tv->color_for = TV_COLOR_COLQ; break; - case 's': tv->no_skip = !tv->no_skip; break; - case 'r': tv->show_name = !tv->show_name; break; - case KEY_LEFT: - case 'h': --pos; break; - case KEY_RIGHT: - case 'l': ++pos; break; - case KEY_SLEFT: - case 'H': pos -= 20; break; - case KEY_SRIGHT: - case 'L': pos += 20; break; - case '.': tv->is_dot = !tv->is_dot; break; - case 'N': tv->base_for = TV_BASE_NUCL; break; - case 'C': tv->base_for = TV_BASE_COLOR_SPACE; break; - case 'i': tv->ins = !tv->ins; break; - case '\010': pos -= 1000; break; - case '\014': pos += 1000; break; - case ' ': pos += tv->mcol; break; - case KEY_UP: - case 'j': --tv->row_shift; break; - case KEY_DOWN: - case 'k': ++tv->row_shift; break; - case KEY_BACKSPACE: - case '\177': pos -= tv->mcol; break; - case KEY_RESIZE: getmaxyx(stdscr, tv->mrow, tv->mcol); break; - default: continue; - } - if (pos < 0) pos = 0; - if (tv->row_shift < 0) tv->row_shift = 0; - tv_draw_aln(tv, tid, pos); - } -end_loop: - return; -} - -int bam_tview_main(int argc, char *argv[]) -{ - tview_t *tv; - if (argc == 1) { - fprintf(stderr, "Usage: bamtk tview [ref.fasta]\n"); - return 1; - } - tv = tv_init(argv[1], (argc == 2)? 0 : argv[2]); - tv_draw_aln(tv, 0, 0); - tv_loop(tv); - tv_destroy(tv); - return 0; -} -#else // #ifdef _HAVE_CURSES -#include -#warning "No curses library is available; tview is disabled." -int bam_tview_main(int argc, char *argv[]) -{ - fprintf(stderr, "[bam_tview_main] The ncurses library is unavailable; tview is not compiled.\n"); - return 1; -} -#endif // #ifdef _HAVE_CURSES