bam_stat.c

   1 #include <unistd.h>
   2 #include <assert.h>
   3 #include "bam.h"
   4
   5 typedef struct {
   6         long long n_reads[2], n_mapped[2], n_pair_all[2], n_pair_map[2], n_pair_good[2];
   7         long long n_sgltn[2], n_read1[2], n_read2[2];
   8         long long n_dup[2];
   9         long long n_diffchr[2], n_diffhigh[2];
  10 } bam_flagstat_t;
  11
  12 #define flagstat_loop(s, c) do {                                                                                \
  13                 int w = ((c)->flag & BAM_FQCFAIL)? 1 : 0;                                               \
  14                 ++(s)->n_reads[w];                                                                                              \
  15                 if ((c)->flag & BAM_FPAIRED) {                                                                  \
  16                         ++(s)->n_pair_all[w];                                                                           \
  17                         if ((c)->flag & BAM_FPROPER_PAIR) ++(s)->n_pair_good[w];        \
  18                         if ((c)->flag & BAM_FREAD1) ++(s)->n_read1[w];                          \
  19                         if ((c)->flag & BAM_FREAD2) ++(s)->n_read2[w];                          \
  20                         if (((c)->flag & BAM_FMUNMAP) && !((c)->flag & BAM_FUNMAP)) ++(s)->n_sgltn[w];  \
  21                         if (!((c)->flag & BAM_FUNMAP) && !((c)->flag & BAM_FMUNMAP)) { \
  22                                 ++(s)->n_pair_map[w];                                                                   \
  23                                 if ((c)->mtid != (c)->tid) {                                                    \
  24                                         ++(s)->n_diffchr[w];                                                            \
  25                                         if ((c)->qual >= 5) ++(s)->n_diffhigh[w];                       \
  26                                 }                                                                                                               \
  27                         }                                                                                                                       \
  28                 }                                                                                                                               \
  29                 if (!((c)->flag & BAM_FUNMAP)) ++(s)->n_mapped[w];                              \
  30                 if ((c)->flag & BAM_FDUP) ++(s)->n_dup[w];                                              \
  31         } while (0)
  32
  33 bam_flagstat_t *bam_flagstat_core(bamFile fp)
  34 {
  35         bam_flagstat_t *s;
  36         bam1_t *b;
  37         bam1_core_t *c;
  38         int ret;
  39         s = (bam_flagstat_t*)calloc(1, sizeof(bam_flagstat_t));
  40         b = bam_init1();
  41         c = &b->core;
  42         while ((ret = bam_read1(fp, b)) >= 0)
  43                 flagstat_loop(s, c);
  44         bam_destroy1(b);
  45         if (ret != -1)
  46                 fprintf(stderr, "[bam_flagstat_core] Truncated file? Continue anyway.\n");
  47         return s;
  48 }
  49 int bam_flagstat(int argc, char *argv[])
  50 {
  51         bamFile fp;
  52         bam_header_t *header;
  53         bam_flagstat_t *s;
  54         if (argc == optind) {
  55                 fprintf(stderr, "Usage: samtools flagstat <in.bam>\n");
  56                 return 1;
  57         }
  58         fp = strcmp(argv[optind], "-")? bam_open(argv[optind], "r") : bam_dopen(fileno(stdin), "r");
  59         assert(fp);
  60         header = bam_header_read(fp);
  61         s = bam_flagstat_core(fp);
  62         printf("%lld + %lld in total (QC-passed reads + QC-failed reads)\n", s->n_reads[0], s->n_reads[1]);
  63         printf("%lld + %lld duplicates\n", s->n_dup[0], s->n_dup[1]);
  64         printf("%lld + %lld mapped (%.2f%%:%.2f%%)\n", s->n_mapped[0], s->n_mapped[1], (float)s->n_mapped[0] / s->n_reads[0] * 100.0, (float)s->n_mapped[1] / s->n_reads[1] * 100.0);
  65         printf("%lld + %lld paired in sequencing\n", s->n_pair_all[0], s->n_pair_all[1]);
  66         printf("%lld + %lld read1\n", s->n_read1[0], s->n_read1[1]);
  67         printf("%lld + %lld read2\n", s->n_read2[0], s->n_read2[1]);
  68         printf("%lld + %lld properly paired (%.2f%%:%.2f%%)\n", s->n_pair_good[0], s->n_pair_good[1], (float)s->n_pair_good[0] / s->n_pair_all[0] * 100.0, (float)s->n_pair_good[1] / s->n_pair_all[1] * 100.0);
  69         printf("%lld + %lld with itself and mate mapped\n", s->n_pair_map[0], s->n_pair_map[1]);
  70         printf("%lld + %lld singletons (%.2f%%:%.2f%%)\n", s->n_sgltn[0], s->n_sgltn[1], (float)s->n_sgltn[0] / s->n_pair_all[0] * 100.0, (float)s->n_sgltn[1] / s->n_pair_all[1] * 100.0);
  71         printf("%lld + %lld with mate mapped to a different chr\n", s->n_diffchr[0], s->n_diffchr[1]);
  72         printf("%lld + %lld with mate mapped to a different chr (mapQ>=5)\n", s->n_diffhigh[0], s->n_diffhigh[1]);
  73         free(s);
  74         bam_header_destroy(header);
  75         bam_close(fp);
  76         return 0;
  77 }