#include <math.h>
#include "sam.h"
#include "khash.h"
+#include "klist.h"
-typedef struct {
- int n, m;
- int *a;
-} listelem_t;
-
-KHASH_MAP_INIT_INT(32, listelem_t)
-
-#define BLOCK_SIZE 65536
+#define QUEUE_CLEAR_SIZE 0x100000
+#define MAX_POS 0x7fffffff
typedef struct {
+ int endpos;
+ uint32_t score:31, discarded:1;
bam1_t *b;
- int rpos, score;
-} elem_t;
+} elem_t, *elem_p;
+#define __free_elem(p) bam_destroy1((p)->data.b)
+KLIST_INIT(q, elem_t, __free_elem)
+typedef klist_t(q) queue_t;
+
+KHASH_MAP_INIT_INT(best, elem_p)
+typedef khash_t(best) besthash_t;
typedef struct {
- int n, max, x;
- elem_t *buf;
-} buffer_t;
+ uint64_t n_checked, n_removed;
+ besthash_t *left, *rght;
+} lib_aux_t;
+KHASH_MAP_INIT_STR(lib, lib_aux_t)
-static int fill_buf(samfile_t *in, buffer_t *buf)
+static lib_aux_t *get_aux(khash_t(lib) *aux, const char *lib)
{
- int i, ret, last_tid, min_rpos = 0x7fffffff, capacity;
- bam1_t *b = bam_init1();
- bam1_core_t *c = &b->core;
- // squeeze out the empty cells at the beginning
- for (i = 0; i < buf->n; ++i)
- if (buf->buf[i].b) break;
- if (i < buf->n) { // squeeze
- if (i > 0) {
- memmove(buf->buf, buf->buf + i, sizeof(elem_t) * (buf->n - i));
- buf->n = buf->n - i;
- }
- } else buf->n = 0;
- // calculate min_rpos
- for (i = 0; i < buf->n; ++i) {
- elem_t *e = buf->buf + i;
- if (e->b && e->rpos >= 0 && e->rpos < min_rpos)
- min_rpos = buf->buf[i].rpos;
- }
- // fill the buffer
- buf->x = -1;
- last_tid = buf->n? buf->buf[0].b->core.tid : -1;
- capacity = buf->n + BLOCK_SIZE;
- while ((ret = samread(in, b)) >= 0) {
- elem_t *e;
- uint8_t *qual = bam1_qual(b);
- int is_mapped;
- if (last_tid < 0) last_tid = c->tid;
- if (c->tid != last_tid) {
- if (buf->x < 0) buf->x = buf->n;
- }
- if (buf->n >= buf->max) { // enlarge
- buf->max = buf->max? buf->max<<1 : 8;
- buf->buf = (elem_t*)realloc(buf->buf, sizeof(elem_t) * buf->max);
- }
- e = &buf->buf[buf->n++];
- e->b = bam_dup1(b);
- e->rpos = -1; e->score = 0;
- for (i = 0; i < c->l_qseq; ++i) e->score += qual[i] + 1;
- e->score = (double)e->score / sqrt(c->l_qseq + 1);
- is_mapped = (c->tid < 0 || c->tid >= in->header->n_targets || (c->flag&BAM_FUNMAP))? 0 : 1;
- if (!is_mapped) e->score = -1;
- if (is_mapped && (c->flag & BAM_FREVERSE)) {
- e->rpos = b->core.pos + bam_calend(&b->core, bam1_cigar(b));
- if (min_rpos > e->rpos) min_rpos = e->rpos;
- }
- if (buf->n >= capacity) {
- if (is_mapped && c->pos <= min_rpos) capacity += BLOCK_SIZE;
- else break;
- }
- }
- if (ret >= 0 && buf->x < 0) buf->x = buf->n;
- bam_destroy1(b);
- return buf->n;
+ khint_t k = kh_get(lib, aux, lib);
+ if (k == kh_end(aux)) {
+ int ret;
+ char *p = strdup(lib);
+ lib_aux_t *q;
+ k = kh_put(lib, aux, p, &ret);
+ q = &kh_val(aux, k);
+ q->left = kh_init(best);
+ q->rght = kh_init(best);
+ q->n_checked = q->n_removed = 0;
+ return q;
+ } else return &kh_val(aux, k);
+}
+
+static inline int sum_qual(const bam1_t *b)
+{
+ int i, q;
+ uint8_t *qual = bam1_qual(b);
+ for (i = q = 0; i < b->core.l_qseq; ++i) q += qual[i];
+ return q;
+}
+
+static inline elem_t *push_queue(queue_t *queue, const bam1_t *b, int endpos, int score)
+{
+ elem_t *p = kl_pushp(q, queue);
+ p->discarded = 0;
+ p->endpos = endpos; p->score = score;
+ if (p->b == 0) p->b = bam_init1();
+ bam_copy1(p->b, b);
+ return p;
}
-static void rmdupse_buf(buffer_t *buf)
+static void clear_besthash(besthash_t *h, int32_t pos)
{
- khash_t(32) *h;
- uint32_t key;
khint_t k;
- int mpos, i, upper;
- listelem_t *p;
- mpos = 0x7fffffff;
- mpos = (buf->x == buf->n)? buf->buf[buf->x-1].b->core.pos : 0x7fffffff;
- upper = (buf->x < 0)? buf->n : buf->x;
- // fill the hash table
- h = kh_init(32);
- for (i = 0; i < upper; ++i) {
- elem_t *e = buf->buf + i;
- int ret;
- if (e->score < 0) continue;
- if (e->rpos >= 0) {
- if (e->rpos <= mpos) key = (uint32_t)e->rpos<<1 | 1;
- else continue;
- } else {
- if (e->b->core.pos < mpos) key = (uint32_t)e->b->core.pos<<1;
- else continue;
- }
- k = kh_put(32, h, key, &ret);
- p = &kh_val(h, k);
- if (ret == 0) { // present in the hash table
- if (p->n == p->m) {
- p->m <<= 1;
- p->a = (int*)realloc(p->a, p->m * sizeof(int));
+ for (k = kh_begin(h); k != kh_end(h); ++k)
+ if (kh_exist(h, k) && kh_val(h, k)->endpos <= pos)
+ kh_del(best, h, k);
+}
+
+static void dump_alignment(samfile_t *out, queue_t *queue, int32_t pos, khash_t(lib) *h)
+{
+ if (queue->size > QUEUE_CLEAR_SIZE || pos == MAX_POS) {
+ khint_t k;
+ while (1) {
+ elem_t *q;
+ if (queue->head == queue->tail) break;
+ q = &kl_val(queue->head);
+ if (q->discarded) {
+ q->b->data_len = 0;
+ kl_shift(q, queue, 0);
+ continue;
}
- p->a[p->n++] = i;
- } else {
- p->m = p->n = 1;
- p->a = (int*)calloc(p->m, sizeof(int));
- p->a[0] = i;
+ if ((q->b->core.flag&BAM_FREVERSE) && q->endpos > pos) break;
+ samwrite(out, q->b);
+ q->b->data_len = 0;
+ kl_shift(q, queue, 0);
}
- }
- // rmdup
- for (k = kh_begin(h); k < kh_end(h); ++k) {
- if (kh_exist(h, k)) {
- int max, maxi;
- p = &kh_val(h, k);
- // get the max
- for (i = max = 0, maxi = -1; i < p->n; ++i) {
- if (buf->buf[p->a[i]].score > max) {
- max = buf->buf[p->a[i]].score;
- maxi = i;
- }
- }
- // mark the elements
- for (i = 0; i < p->n; ++i) {
- buf->buf[p->a[i]].score = -1;
- if (i != maxi) {
- bam_destroy1(buf->buf[p->a[i]].b);
- buf->buf[p->a[i]].b = 0;
- }
+ for (k = kh_begin(h); k != kh_end(h); ++k) {
+ if (kh_exist(h, k)) {
+ clear_besthash(kh_val(h, k).left, pos);
+ clear_besthash(kh_val(h, k).rght, pos);
}
- // free
- free(p->a);
}
}
- kh_destroy(32, h);
}
-static void dump_buf(buffer_t *buf, samfile_t *out)
+void bam_rmdupse_core(samfile_t *in, samfile_t *out, int force_se)
{
- int i;
- for (i = 0; i < buf->n; ++i) {
- elem_t *e = buf->buf + i;
- if (e->score != -1) break;
- if (e->b) {
- samwrite(out, e->b);
- bam_destroy1(e->b);
- e->b = 0;
+ bam1_t *b;
+ queue_t *queue;
+ khint_t k;
+ int last_tid = -2;
+ khash_t(lib) *aux;
+
+ aux = kh_init(lib);
+ b = bam_init1();
+ queue = kl_init(q);
+ while (samread(in, b) >= 0) {
+ bam1_core_t *c = &b->core;
+ int endpos = bam_calend(c, bam1_cigar(b));
+ int score = sum_qual(b);
+
+ if (last_tid != c->tid) {
+ if (last_tid >= 0) dump_alignment(out, queue, MAX_POS, aux);
+ last_tid = c->tid;
+ } else dump_alignment(out, queue, c->pos, aux);
+ if ((c->flag&BAM_FUNMAP) || ((c->flag&BAM_FPAIRED) && !force_se)) {
+ push_queue(queue, b, endpos, score);
+ } else {
+ const char *lib;
+ lib_aux_t *q;
+ besthash_t *h;
+ uint32_t key;
+ int ret;
+ lib = bam_get_library(in->header, b);
+ q = lib? get_aux(aux, lib) : get_aux(aux, "\t");
+ ++q->n_checked;
+ h = (c->flag&BAM_FREVERSE)? q->rght : q->left;
+ key = (c->flag&BAM_FREVERSE)? endpos : c->pos;
+ k = kh_put(best, h, key, &ret);
+ if (ret == 0) { // in the hash table
+ elem_t *p = kh_val(h, k);
+ ++q->n_removed;
+ if (p->score < score) {
+ if (c->flag&BAM_FREVERSE) { // mark "discarded" and push the queue
+ p->discarded = 1;
+ kh_val(h, k) = push_queue(queue, b, endpos, score);
+ } else { // replace
+ p->score = score; p->endpos = endpos;
+ bam_copy1(p->b, b);
+ }
+ } // otherwise, discard the alignment
+ } else kh_val(h, k) = push_queue(queue, b, endpos, score);
}
}
-}
+ dump_alignment(out, queue, MAX_POS, aux);
-int bam_rmdupse(int argc, char *argv[])
-{
- samfile_t *in, *out;
- buffer_t *buf;
- if (argc < 3) {
- fprintf(stderr, "Usage: samtools rmdupse <in.bam> <out.bam>\n\n");
- fprintf(stderr, "Note: Picard is recommended for this task.\n");
- return 1;
- }
- buf = calloc(1, sizeof(buffer_t));
- in = samopen(argv[1], "rb", 0);
- out = samopen(argv[2], "wb", in->header);
- while (fill_buf(in, buf)) {
- rmdupse_buf(buf);
- dump_buf(buf, out);
+ for (k = kh_begin(aux); k != kh_end(aux); ++k) {
+ if (kh_exist(aux, k)) {
+ lib_aux_t *q = &kh_val(aux, k);
+ fprintf(stderr, "[bam_rmdupse_core] %lld / %lld = %.4lf in library '%s'\n", (long long)q->n_removed,
+ (long long)q->n_checked, (double)q->n_removed/q->n_checked, kh_key(aux, k));
+ kh_destroy(best, q->left); kh_destroy(best, q->rght);
+ free((char*)kh_key(aux, k));
+ }
}
- samclose(in); samclose(out);
- free(buf->buf); free(buf);
- return 0;
+ kh_destroy(lib, aux);
+ bam_destroy1(b);
+ kl_destroy(q, queue);
}
projects / samtools.git / blobdiff