X-Git-Url: http://woldlab.caltech.edu/gitweb/?p=samtools.git;a=blobdiff_plain;f=bam.h;h=eef2ea9853f25966c5a40c45a86272b78a507fb2;hp=8e26ea69d3b7c6b008aba6112052310a4072b08a;hb=016b50ab60e879a0b8f81cb76ce11ea360a03d4a;hpb=67801bf17aa6495879da63ce77a2014759fabc16 diff --git a/bam.h b/bam.h index 8e26ea6..eef2ea9 100644 --- a/bam.h +++ b/bam.h @@ -1,6 +1,6 @@ /* The MIT License - Copyright (c) 2008 Genome Research Ltd (GRL). + Copyright (c) 2008-2010 Genome Research Ltd (GRL). Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the @@ -230,7 +230,7 @@ typedef struct __bam_iter_t *bam_iter_t; @param b pointer to an alignment @return pointer to quality string */ -#define bam1_qual(b) ((b)->data + (b)->core.n_cigar*4 + (b)->core.l_qname + ((b)->core.l_qseq + 1)/2) +#define bam1_qual(b) ((b)->data + (b)->core.n_cigar*4 + (b)->core.l_qname + (((b)->core.l_qseq + 1)>>1)) /*! @function @abstract Get a base on read @@ -455,6 +455,21 @@ extern "C" { char *bam_format1_core(const bam_header_t *header, const bam1_t *b, int of); + /*! + @abstract Check whether a BAM record is plausibly valid + @param header associated header structure, or NULL if unavailable + @param b alignment to validate + @return 0 if the alignment is invalid; non-zero otherwise + + @discussion Simple consistency check of some of the fields of the + alignment record. If the header is provided, several additional checks + are made. Not all fields are checked, so a non-zero result is not a + guarantee that the record is valid. However it is usually good enough + to detect when bam_seek() has been called with a virtual file offset + that is not the offset of an alignment record. + */ + int bam_validate1(const bam_header_t *header, const bam1_t *b); + const char *bam_get_library(bam_header_t *header, const bam1_t *b); @@ -480,7 +495,7 @@ extern "C" { bam1_t *b; int32_t qpos; int indel, level; - uint32_t is_del:1, is_head:1, is_tail:1; + uint32_t is_del:1, is_head:1, is_tail:1, is_refskip:1, aux:28; } bam_pileup1_t; typedef int (*bam_plp_auto_f)(void *data, bam1_t *b); @@ -493,6 +508,7 @@ extern "C" { const bam_pileup1_t *bam_plp_next(bam_plp_t iter, int *_tid, int *_pos, int *_n_plp); const bam_pileup1_t *bam_plp_auto(bam_plp_t iter, int *_tid, int *_pos, int *_n_plp); void bam_plp_set_mask(bam_plp_t iter, int mask); + void bam_plp_set_maxcnt(bam_plp_t iter, int maxcnt); void bam_plp_reset(bam_plp_t iter); void bam_plp_destroy(bam_plp_t iter); @@ -501,6 +517,7 @@ extern "C" { bam_mplp_t bam_mplp_init(int n, bam_plp_auto_f func, void **data); void bam_mplp_destroy(bam_mplp_t iter); + void bam_mplp_set_maxcnt(bam_mplp_t iter, int maxcnt); int bam_mplp_auto(bam_mplp_t iter, int *_tid, int *_pos, int *n_plp, const bam_pileup1_t **plp); /*! @typedef @@ -693,8 +710,8 @@ static inline bam1_t *bam_copy1(bam1_t *bdst, const bam1_t *bsrc) { uint8_t *data = bdst->data; int m_data = bdst->m_data; // backup data and m_data - if (m_data < bsrc->m_data) { // double the capacity - m_data = bsrc->m_data; kroundup32(m_data); + if (m_data < bsrc->data_len) { // double the capacity + m_data = bsrc->data_len; kroundup32(m_data); data = (uint8_t*)realloc(data, m_data); } memcpy(data, bsrc->data, bsrc->data_len); // copy var-len data