X-Git-Url: http://woldlab.caltech.edu/gitweb/?p=samtools.git;a=blobdiff_plain;f=misc%2Fwgsim_eval.pl;h=f919a0643a0d2950d8f817c6213a3183f4f9a6dc;hp=99e2ac93b1c73fd37525bc963fcc4959a571cf9f;hb=HEAD;hpb=b27e00385f41769d03a8cca4dbd71275fc9fa906

diff --git a/misc/wgsim_eval.pl b/misc/wgsim_eval.pl
index 99e2ac9..f919a06 100755
--- a/misc/wgsim_eval.pl
+++ b/misc/wgsim_eval.pl
@@ -1,7 +1,7 @@
 #!/usr/bin/perl -w
 
 # Contact: lh3
-# Version: 0.1.3
+# Version: 0.1.5
 
 use strict;
 use warnings;
@@ -11,16 +11,18 @@ use Getopt::Std;
 exit;
 
 sub wgsim_eval {
-  my %opts;
-  getopts('pc', \%opts);
-  die("Usage: wgsim_eval.pl [-pc] <in.sam>\n") if (@ARGV == 0 && -t STDIN);
-  my (@c0, @c1);
+  my %opts = (g=>5);
+  getopts('pcag:', \%opts);
+  die("Usage: wgsim_eval.pl [-pca] [-g $opts{g}] <in.sam>\n") if (@ARGV == 0 && -t STDIN);
+  my (@c0, @c1, %fnfp);
   my ($max_q, $flag) = (0, 0);
-  my $gap = 5;
+  my $gap = $opts{g};
   $flag |= 1 if (defined $opts{p});
   $flag |= 2 if (defined $opts{c});
   while (<>) {
-	my @t = split;
+	next if (/^\@/);
+	my @t = split("\t");
+	next if (@t < 11);
 	my $line = $_;
 	my ($q, $is_correct, $chr, $left, $rght) = (int($t[4]/10), 1, $t[2], $t[3], $t[3]);
 	$max_q = $q if ($q > $max_q);
@@ -28,8 +30,11 @@ sub wgsim_eval {
 	$_ = $t[5]; s/(\d+)[MDN]/$rght+=$1,'x'/eg;
 	--$rght;
 	# correct for soft clipping
-	$left -= $1 if (/^(\d+)S/);
-	$rght += $1 if (/(\d+)S$/);
+	my ($left0, $rght0) = ($left, $rght);
+	$left -= $1 if (/^(\d+)[SH]/);
+	$rght += $1 if (/(\d+)[SH]$/);
+	$left0 -= $1 if (/(\d+)[SH]$/);
+	$rght0 += $1 if (/^(\d+)[SH]/);
 	# skip unmapped reads
 	next if (($t[1]&0x4) || $chr eq '*');
 	# parse read name and check
@@ -39,19 +44,19 @@ sub wgsim_eval {
 	  } else {
 		if ($flag & 2) {
 		  if (($t[1]&0x40) && !($t[1]&0x10)) { # F3, forward
-			$is_correct = 0 if (abs($2 - $left) > $gap);
+			$is_correct = 0 if (abs($2 - $left) > $gap && abs($2 - $left0) > $gap);
 		  } elsif (($t[1]&0x40) && ($t[1]&0x10)) { # F3, reverse
-			$is_correct = 0 if (abs($3 - $rght) > $gap);
+			$is_correct = 0 if (abs($3 - $rght) > $gap && abs($3 - $rght0) > $gap);
 		  } elsif (($t[1]&0x80) && !($t[1]&0x10)) { # R3, forward
-			$is_correct = 0 if (abs($3 - $left) > $gap);
+			$is_correct = 0 if (abs($3 - $left) > $gap && abs($3 - $left0) > $gap);
 		  } else { # R3, reverse
-			$is_correct = 0 if (abs($2 - $rght) > $gap);
+			$is_correct = 0 if (abs($2 - $rght) > $gap && abs($3 - $rght0) > $gap);
 		  }
 		} else {
 		  if ($t[1] & 0x10) { # reverse
-			$is_correct = 0 if (abs($3 - $rght) > $gap); # in case of indels that are close to the end of a reads
+			$is_correct = 0 if (abs($3 - $rght) > $gap && abs($3 - $rght0) > $gap); # in case of indels that are close to the end of a reads
 		  } else {
-			$is_correct = 0 if (abs($2 - $left) > $gap);
+			$is_correct = 0 if (abs($2 - $left) > $gap && abs($2 - $left0) > $gap);
 		  }
 		}
 	  }
@@ -61,14 +66,26 @@ sub wgsim_eval {
 	}
 	++$c0[$q];
 	++$c1[$q] unless ($is_correct);
+	@{$fnfp{$t[4]}} = (0, 0) unless (defined $fnfp{$t[4]});
+	++$fnfp{$t[4]}[0];
+	++$fnfp{$t[4]}[1] unless ($is_correct);
 	print STDERR $line if (($flag&1) && !$is_correct && $q > 0);
   }
   # print
   my ($cc0, $cc1) = (0, 0);
-  for (my $i = $max_q; $i >= 0; --$i) {
-	$c0[$i] = 0 unless (defined $c0[$i]);
-	$c1[$i] = 0 unless (defined $c1[$i]);
-	$cc0 += $c0[$i]; $cc1 += $c1[$i];
-	printf("%.2dx %12d / %-12d  %12d  %.3e\n", $i, $c1[$i], $c0[$i], $cc0, $cc1/$cc0);
+  if (!defined($opts{a})) {
+	for (my $i = $max_q; $i >= 0; --$i) {
+	  $c0[$i] = 0 unless (defined $c0[$i]);
+	  $c1[$i] = 0 unless (defined $c1[$i]);
+	  $cc0 += $c0[$i]; $cc1 += $c1[$i];
+	  printf("%.2dx %12d / %-12d  %12d  %.3e\n", $i, $c1[$i], $c0[$i], $cc0, $cc1/$cc0) if ($cc0);
+	}
+  } else {
+	for (reverse(sort {$a<=>$b} (keys %fnfp))) {
+	  next if ($_ == 0);
+	  $cc0 += $fnfp{$_}[0];
+	  $cc1 += $fnfp{$_}[1];
+	  print join("\t", $_, $cc0, $cc1), "\n";
+	}
   }
 }