X-Git-Url: http://woldlab.caltech.edu/gitweb/?p=samtools.git;a=blobdiff_plain;f=sam_view.c;h=06dd01a5ffa951dcb950a97681e7c5aca0b88971;hp=113c6c437479237791cee16164d6fbc06cd7af8b;hb=317f5e8dd22cc9e1e5e05fbcaeb3b9aca7447351;hpb=4a17fa7e1f91b2fe04ad334a63fc2b0d5e859d8a diff --git a/sam_view.c b/sam_view.c index 113c6c4..06dd01a 100644 --- a/sam_view.c +++ b/sam_view.c @@ -2,26 +2,45 @@ #include #include #include +#include +#include "sam_header.h" #include "sam.h" #include "faidx.h" static int g_min_mapQ = 0, g_flag_on = 0, g_flag_off = 0; static char *g_library, *g_rg; +static int g_sol2sanger_tbl[128]; + +static void sol2sanger(bam1_t *b) +{ + int l; + uint8_t *qual = bam1_qual(b); + if (g_sol2sanger_tbl[30] == 0) { + for (l = 0; l != 128; ++l) { + g_sol2sanger_tbl[l] = (int)(10.0 * log(1.0 + pow(10.0, (l - 64 + 33) / 10.0)) / log(10.0) + .499); + if (g_sol2sanger_tbl[l] >= 93) g_sol2sanger_tbl[l] = 93; + } + } + for (l = 0; l < b->core.l_qseq; ++l) { + int q = qual[l]; + if (q > 127) q = 127; + qual[l] = g_sol2sanger_tbl[q]; + } +} static inline int __g_skip_aln(const bam_header_t *h, const bam1_t *b) { if (b->core.qual < g_min_mapQ || ((b->core.flag & g_flag_on) != g_flag_on) || (b->core.flag & g_flag_off)) return 1; - if (g_library || g_rg) { + if (g_rg) { uint8_t *s = bam_aux_get(b, "RG"); - if (s) { - if (g_rg && strcmp(g_rg, (char*)(s + 1)) == 0) return 0; - if (g_library) { - const char *p = bam_strmap_get(h->rg2lib, (char*)(s + 1)); - return (p && strcmp(p, g_library) == 0)? 0 : 1; - } return 1; - } else return 1; - } else return 0; + if (s && strcmp(g_rg, (char*)(s + 1)) == 0) return 0; + } + if (g_library) { + const char *p = bam_get_library((bam_header_t*)h, b); + return (p && strcmp(p, g_library) == 0)? 0 : 1; + } + return 0; } // callback function for bam_fetch() @@ -36,15 +55,16 @@ static int usage(int is_long_help); int main_samview(int argc, char *argv[]) { - int c, is_header = 0, is_header_only = 0, is_bamin = 1, ret = 0, is_uncompressed = 0, is_bamout = 0; + int c, is_header = 0, is_header_only = 0, is_bamin = 1, ret = 0, is_uncompressed = 0, is_bamout = 0, slx2sngr = 0; int of_type = BAM_OFDEC, is_long_help = 0; samfile_t *in = 0, *out = 0; char in_mode[5], out_mode[5], *fn_out = 0, *fn_list = 0, *fn_ref = 0; /* parse command-line options */ strcpy(in_mode, "r"); strcpy(out_mode, "w"); - while ((c = getopt(argc, argv, "Sbt:hHo:q:f:F:ul:r:xX?T:")) >= 0) { + while ((c = getopt(argc, argv, "Sbt:hHo:q:f:F:ul:r:xX?T:C")) >= 0) { switch (c) { + case 'C': slx2sngr = 1; break; case 'S': is_bamin = 0; break; case 'b': is_bamout = 1; break; case 't': fn_list = strdup(optarg); is_bamin = 0; break; @@ -96,9 +116,12 @@ int main_samview(int argc, char *argv[]) if (argc == optind + 1) { // convert/print the entire file bam1_t *b = bam_init1(); int r; - while ((r = samread(in, b)) >= 0) // read one alignment from `in' - if (!__g_skip_aln(in->header, b)) + while ((r = samread(in, b)) >= 0) { // read one alignment from `in' + if (!__g_skip_aln(in->header, b)) { + if (slx2sngr) sol2sanger(b); samwrite(out, b); // write the alignment to `out' + } + } if (r < -1) fprintf(stderr, "[main_samview] truncated file.\n"); bam_destroy1(b); } else { // retrieve alignments in specified regions