FastQCFastQC Report
Sun 31 May 2015
15808_ACAGTG_L001_R1_002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15808_ACAGTG_L001_R1_002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCA64960.1624No Hit
CGCCGCCATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCAC51960.12990000000000002No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC44480.1112No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTT44450.111125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTT101500.015.7054851
CGGCCT17450.011.4032651
AATCCG15600.011.3090944
AGTTAC18000.010.67436510
GAATTC34700.010.44494154
TTTGGC17250.010.3260647
CGTAAC18300.010.2650133
TACCAT19400.010.0248329
TCGTAA20200.09.9559262
TAGTTA29900.09.8741589
CGACGT11550.09.54663444
CACGTC11100.09.28866814
CTTAGT25100.09.1508767
ACTTAG34300.09.140446
TTAGTT33600.08.9268918
CGTTAA25550.08.80448814
ACGTTA26350.08.80396613
GGCTAA18300.08.70679910
GGATTG21100.08.66212311
ATTCAA58500.08.4540556
Produced by FastQC (version 0.11.3)