FastQCFastQC Report
Sun 31 May 2015
15808_ACAGTG_L001_R1_003.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15808_ACAGTG_L001_R1_003.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCA61800.1545No Hit
CGCCGCCATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCAC48830.122075No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTT42530.106325No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC41760.1044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTT97700.015.6255261
CGGCCT14000.012.9110411
CCGGTA16850.011.38071312
AATCCG16700.010.91528244
TTTGGC16200.010.7103677
TACCAT17000.010.6018889
GGCTAA16550.010.19071410
GAATTC32150.09.9443394
TAGTTA27000.09.1570929
TCGTAA21000.09.1195852
ACTAAC20550.08.97592543
ATTCAA57800.08.973236
GTTTAG30550.08.7209635
AGTTAC17500.08.7133610
CAAGTA22050.08.66078238
GCCTAC23400.08.6552251
ACATTC38300.08.649984
GAGATA25000.08.6182835
CGTAAC18950.08.6131843
CAGACT14500.08.5549723
Produced by FastQC (version 0.11.3)