FastQCFastQC Report
Sun 31 May 2015
15808_ACAGTG_L001_R1_004.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15808_ACAGTG_L001_R1_004.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCA65590.16397499999999998No Hit
CGCCGCCATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCAC50620.12655No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC44340.11084999999999999No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTT44200.11050000000000001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTT102950.015.4900051
CGGCCT17400.011.7964311
TTTGGC16250.011.4944487
CACGTC9650.010.95524714
AATCCG16350.010.86855544
GAATTC32500.010.3300964
ACTTGC22750.09.49164413
CGTAAC17850.09.4672633
GGCTAA19000.09.35232610
TAGTTA29200.09.32070359
CGTTAA23900.09.31727514
AGGGTC16800.09.23851841
CAGACT16100.09.2367563
AGTTAC19050.09.09163410
ACACGT14450.09.028419513
ATTCAA56250.09.01283556
TCGTAA20350.08.9685472
CGACCA23900.08.94104340
GTAACT26150.08.85955129
ACGTTA25450.08.838213
Produced by FastQC (version 0.11.3)