FastQCFastQC Report
Sun 31 May 2015
15808_ACAGTG_L001_R1_005.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15808_ACAGTG_L001_R1_005.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2604609
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCA37190.14278534705209112No Hit
CGCCGCCATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCAC28770.11045803803949077No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC26850.10308649014113058No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTT26500.10174271838882536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTT66650.015.6577341
GAATTC23350.010.8023944
CGGCCT12200.010.5308421
TAGTTA19850.010.4225449
TTTGGC10150.09.7500787
TACCAT13400.09.7335139
AGTTAC13900.09.70676510
TGTCGA12850.09.44975120
ACTTAG24500.09.3752156
CAGACT12300.09.1549033
CACGTC7450.09.05530514
CTTAGT18500.08.9966637
TTAGTT23300.08.9771848
ATTCAA38850.08.5786286
ACGTTA15750.08.56660613
CTCGAC12250.08.44406321
TTGTAC14400.08.43291527
GGGGAT28550.08.3615862
CTTACC10250.08.35489433
TCATTC16850.08.27395542
Produced by FastQC (version 0.11.3)