FastQCFastQC Report
Sun 31 May 2015
15808_ACAGTG_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15808_ACAGTG_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCA72220.18055000000000002No Hit
CGCCGCCATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCAC57250.143125No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC51790.12947499999999998No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTT49860.12465No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC44310.110775No Hit
CCCGGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCAT42300.10575000000000001No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG41440.1036No Hit
GCTCGAGTTGCCCCTTTGCCCGATCCCGGAAGATACGCTCTAAGCTCGGC41140.10285No Hit
GGGACATTCAAATGAGCATCCTAGTCACCGCGTTTAAAATGAACCTGCCG40710.101775No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA40330.100825No Hit
GCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCG40120.1003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTT102000.016.1317041
CGGCCT15000.012.75530051
AATCCG15000.011.99973744
TTTGGC16200.011.9438837
AGTTAC17700.011.82147910
CACGTC10600.011.46172614
GAATTC35400.010.9981334
TACCAT19950.010.9393377
GGCTAA15900.010.75421210
TAGTTA29250.010.3841289
AGTGAT12450.09.93941736
TCGTAT12500.09.89978343
TCGTAA19350.09.88612
ACACGT15100.09.68497713
CGTAAC18850.09.6707623
TCATTC25700.09.630542
CGTTAA23550.09.45815614
CTTAGT28900.09.4199727
ACTTAG38700.09.3600256
CTTACC13600.09.09895133
Produced by FastQC (version 0.11.3)