FastQCFastQC Report
Sun 31 May 2015
15808_ACAGTG_L002_R1_002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15808_ACAGTG_L002_R1_002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCA70100.17525No Hit
CGCCGCCATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCAC56200.1405No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC50220.12555000000000002No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTT49340.12335No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC41910.10477500000000001No Hit
CCCGGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCAT40690.101725No Hit
GCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCG40450.10112499999999999No Hit
GTCGGTTTAGCTGAGATTGCACCGTTGTTATAATCGACCCGGGCTGCGAC40300.10074999999999999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTT100150.017.1851861
TTTGGC14500.012.41867
AGTTAC17850.011.9714910
CGGCCT16600.011.9579091
TAGTTA28700.011.52150259
AATCCG15050.010.91034144
GGCGTA7750.010.7691321
GGCTAA16150.010.44603610
GAATTC31950.010.2957164
TACCAT19800.09.8838869
CACGTC10300.09.608414
CGTAAC17650.09.5739513
TTAGTT34700.09.5326488
ACTTAG39100.09.3348526
GTTACA24150.09.22106111
CATACC11050.09.1753973
AGGGTC18650.08.92537741
CTTAGT29600.08.8970257
TCGTAA20550.08.88071252
GCACAT17100.08.68192638
Produced by FastQC (version 0.11.3)