FastQCFastQC Report
Sun 31 May 2015
15808_ACAGTG_L002_R1_003.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15808_ACAGTG_L002_R1_003.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCA71650.179125No Hit
CGCCGCCATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCAC57350.143375No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTT51000.1275No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC50520.1263No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC42330.10582499999999999No Hit
CCCGGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCAT41410.10352499999999999No Hit
GCTCGAGTTGCCCCTTTGCCCGATCCCGGAAGATACGCTCTAAGCTCGGC40580.10145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTT99050.015.4681831
TTTGGC16300.013.5262337
CACGTC9550.012.95647314
GGCTAA16500.012.27117310
CGGCCT17900.012.2096611
AATCCG16050.010.93374144
AGCTAG21350.010.5421836
GAATTC33300.010.478854
CGTAAC17900.010.3130473
TCGTAT11600.010.27951343
TCGTAA20800.010.1739752
ATTCAA60900.09.7569716
AGTGAT11600.09.69704836
AATGAA26750.09.41960238
TAGTTA30350.09.1174619
ACGTTA25550.09.06930113
ATCGAC29650.09.02943633
GGATTG19950.09.02141311
CCGGTA17450.08.89572112
TACCAT19300.08.8592027
Produced by FastQC (version 0.11.3)