FastQCFastQC Report
Sun 31 May 2015
15808_ACAGTG_L002_R1_004.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15808_ACAGTG_L002_R1_004.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCA69170.17292500000000002No Hit
CGCCGCCATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCAC54580.13645000000000002No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC47520.1188No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTT46820.11705000000000002No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC40980.10245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTT101600.016.07071
CGGCCT16550.012.9275471
AATCCG16400.011.11240444
GGCTAA17250.010.95461910
TTTGGC17450.010.7002817
CGTAAC18900.010.4814473
CACGTC9100.010.13560314
AGTTAC18200.010.01199810
TACCAT20050.09.8735979
TCGTAA21150.09.2599632
AGCTAG24000.09.0968336
GAACCG25400.08.85761532
TAATCC22900.08.841377
CCGGTA16550.08.83532412
TCGTAT10700.08.83145743
GAATTC37250.08.8232174
ATCCGG14950.08.57828542
TAAATG22850.08.56557330
GTAACC23050.08.4966694
GTGAAC26850.08.46253530
Produced by FastQC (version 0.11.3)