FastQCFastQC Report
Sun 31 May 2015
15808_ACAGTG_L002_R1_005.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15808_ACAGTG_L002_R1_005.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2717129
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCA44230.16278211303180673No Hit
CGCCGCCATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCAC33940.12491125743385757No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTT31620.11637283323684669No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC30660.1128396921898077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTT69450.015.7236841
CGGCCT11450.011.4053161
GAATTC24200.011.0678814
TACCAT13600.010.4200719
TTTGGC10500.010.2826487
CACGTC7900.09.9657514
GAACCG18750.09.59978832
TTATAG14800.09.5810186
TCGTAT8550.09.47190643
GTGAAC19200.09.37272330
TAGTTA20350.09.1745069
AGTGAT8650.09.10367836
GGTTGC20600.09.06316614
CGTAAC12700.09.0385613
GGATCC9600.08.9124511
GAGATA18200.08.9041813
GCCTAA20400.08.82593318
TGTCGA13600.08.77093320
CATTGT9000.08.75254620
CCTAAG20750.08.67722219
Produced by FastQC (version 0.11.3)