FastQCFastQC Report
Sun 31 May 2015
15809_GCCAAT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15809_GCCAAT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCA45290.113225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTT106600.012.6032161
CGTAAC16450.010.957323
ACGTTA19250.08.64718813
TCGTAA23700.08.1758022
ATCGAC26450.07.654040333
GTAACC26200.07.6536564
CGGGGT49200.07.4223641
TCGACC25900.07.382323734
CGAATT38700.07.33567053
GAATTC43050.07.32715034
TAACGT12350.07.29748634
GACGTT23900.07.247142312
CGTTAA22800.07.20214614
CGACCC26550.07.20158935
CTCGTA31600.07.13354251
ATAAGC29100.07.11153468
GTCGAC23750.07.10348726
CGCGAA20700.07.07840441
GAATCG23250.07.06266243
AATCTA34500.07.05300245
Produced by FastQC (version 0.11.3)