FastQCFastQC Report
Sun 31 May 2015
15809_GCCAAT_L001_R1_002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15809_GCCAAT_L001_R1_002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCA43230.10807499999999999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTT108950.012.4594931
ACGTTA19150.010.006989513
CGACGT8850.09.7292244
CGTAAC13250.09.5219233
AATCCG15500.08.83113944
CGTTAA22300.08.698359514
CGCAAA23950.08.58665530
GAATCG23800.08.1633443
GACGTT23850.08.1329512
GTCGGG27250.08.045181
GAATTC40500.07.7060134
ATTCAA54550.07.5778646
ACTTAG36250.07.37865736
GTAACC23450.07.26797684
TCGTAA21450.07.2233132
CTTACC16300.07.178417733
ATCCGC22450.06.975206445
ACGAAT24400.06.89428852
GTAACT29900.06.79978129
CTCGTA26800.06.7755741