FastQCFastQC Report
Sun 31 May 2015
15809_GCCAAT_L001_R1_003.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15809_GCCAAT_L001_R1_003.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTT102650.011.4194721
CGTAAC15000.09.1770443
AATCCG13950.09.06191444
ATTCAA56000.08.0777386
ACGTTA20850.08.06342313
CGACGT9800.07.784095344
TCGTAA22150.07.39845562
CGTTAA23100.07.07862714
GAATCG23450.07.06439443
GTCGGG24350.07.00544641
GCGGGT23350.06.9308261
GTAACC24700.06.9152554
CTCGTA30800.06.88749361
CTTGCG20750.06.88139214
TCGACC23500.06.86462734
CGAATT35400.06.78958033
CGGGTT23850.06.77948672
GAATTC40350.06.7501274
CGCGAA19150.06.73788171
TAGGTG33850.06.73564616
Produced by FastQC (version 0.11.3)