FastQCFastQC Report
Sun 31 May 2015
15809_GCCAAT_L001_R1_004.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15809_GCCAAT_L001_R1_004.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCA43290.10822500000000002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTT112550.012.4627791
CGTAAC14250.08.8547973
ACGTTA23600.08.76862313
ATTCAA60100.08.1356216
GACGTT27200.08.02125212
TCGTAA22850.07.88876872
AATCCG15000.07.79723344
GAATCG24950.07.753050343
CGTTAA26150.07.7416214
CGCAAA26150.07.740942530
GAATTC44950.07.71963024
GTCCGC41550.07.3813851
ACTTAG32550.07.1992596
GTACGT7551.3960744E-107.1685991
CACGTC11750.07.08312414
CGGCCT21350.06.9713371
CGAATT38200.06.84226853
TAGTTA33300.06.6870679
TCGACC24900.06.684876434
CGGGGG61550.06.63163331
Produced by FastQC (version 0.11.3)