FastQCFastQC Report
Sun 31 May 2015
15809_GCCAAT_L001_R1_005.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15809_GCCAAT_L001_R1_005.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258595
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTT32600.010.9954491
AATCCG4705.857146E-109.08679144
CGTAAC4901.1928023E-98.7342923
TAACGT4054.3055525E-78.3426964
CGTATC5051.8383162E-88.0288123
AGTTGC9600.07.97643386
CGATCA8750.07.741754534
AACGAA21400.07.68263446
CTGGGA15100.07.61424261
GAATTC13100.07.56574344
ATTCAA18550.07.4060566
CGGCCT6402.2478162E-97.3972921
GATCTA9150.07.3841625
AACCGG7652.3192115E-117.37912333
TACGTA2750.00275156277.3707365
AACGTC4959.444507E-77.279745
ACGTTA8404.092726E-127.225310313
TTACTC8404.092726E-127.225310313
CGTTAA9104.5474735E-137.16355614
TTACGG5651.2499686E-77.161085620
Produced by FastQC (version 0.11.3)