FastQCFastQC Report
Sun 31 May 2015
15809_GCCAAT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15809_GCCAAT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCA45640.11410000000000001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTT105650.012.910261
AATCCG14250.09.78916944
CGTAAC15600.08.9441543
GTCGGG25050.07.72720771
ATACGT9100.07.664884642
CACGTC12050.07.655267714
ATCCGC19750.07.404833345
CGAATT39300.07.38702063
AATGCG28850.07.330703313
TCGTAT11700.07.307464643
CGACCA21900.07.191826340
ATTCAA57500.07.1610056
CTTGCG24050.07.10987714
TCGTAA22350.07.04843472
GTCGAC23350.07.034306526
CGGGTT25000.06.8414122
TAACGT13600.06.78448874
CTTAGT26900.06.7752217
ACGTTA20650.06.755153713
ACTTAG34000.06.7501286
Produced by FastQC (version 0.11.3)