FastQCFastQC Report
Sun 31 May 2015
15809_GCCAAT_L002_R1_002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15809_GCCAAT_L002_R1_002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCA44600.11150000000000002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTT110850.012.8304151
CGTAAC13650.010.569563
AATCCG14300.07.86380244
ACTTAG33450.07.8173686
AGTTAC24200.07.714446510
ACGTTA23100.07.593711413
CTTGCG22400.07.52982214
GTAACC24950.07.4992384
TAGTTA35650.07.44769679
TCGTAA22100.07.4462942
ATTCAA60800.07.37816246
CGTTAA24750.07.360058314
CTTAGT27050.07.32547047
GAATTC43100.07.27021274
GGGAGA58350.07.15758751
CGGGGT49600.07.10033371
GACGTT27100.07.053855412
CTCGTA30500.06.88365271
GTCGGG24650.06.86878871
GGGGAT44800.06.74273972
Produced by FastQC (version 0.11.3)