FastQCFastQC Report
Sun 31 May 2015
15809_GCCAAT_L002_R1_003.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15809_GCCAAT_L002_R1_003.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCA46670.116675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTT108200.012.6783441
AATCCG13850.010.23404244
CGTAAC16500.09.9606713
ACGTTA21600.09.26958213
CGTTAA23300.08.78636614
GAATCG22800.08.78280743
TCGTAA23300.08.5030832
ATTCAA56900.08.30499656
CGCAAA25350.07.89863830
GTCGGG25750.07.69800141
TAGTTA36400.07.66380029
GACGTT26650.07.59747312
CGACCC25500.07.587210735
TCGACC24950.07.48395934
ATCCGC19900.07.461864545
GAATTC45650.07.39775944
CGGGGT48600.07.32307241
ACTTAG36500.07.27480946
GTAACC24900.07.23336464
ATCGAC25450.07.160402333
Produced by FastQC (version 0.11.3)