FastQCFastQC Report
Sun 31 May 2015
15809_GCCAAT_L002_R1_004.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15809_GCCAAT_L002_R1_004.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCA43050.107625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTT108200.012.8238391
ACGTTA24150.09.03497813
ATTCAA60250.08.856136
AATCCG14500.08.84380344
CGTAAC16550.08.8427993
CGCAAA26150.08.77483530
GAATCG24650.08.76155343
CGTTAA25950.08.5816414
GACGTT27050.08.31582212
GAATTC44150.07.700534
CTTGCG21450.07.655402714
GTCGGG27500.07.61754271
TATCGA19550.07.59464825
CCCGAC21500.07.539952
GTAACC24250.07.5205174
CGACCA21050.07.37499540
TCGAGT44900.07.3211772
TTATCG20450.07.26059124
TCGTAA23600.07.1552312
TCGTTC34050.06.93639479
Produced by FastQC (version 0.11.3)