FastQCFastQC Report
Sun 31 May 2015
15809_GCCAAT_L002_R1_005.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename15809_GCCAAT_L002_R1_005.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1392778
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAAACGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCA14240.102241706862113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTT35800.012.3307191
AATCCG4951.546141E-119.54159444
CGACGT3556.717137E-78.8696544
TTTGCG4654.611593E-98.71084540
GTTCGA3953.014884E-78.5528231
CGAATT12150.08.3392443
ATACGT2753.0481414E-48.18291642
TTCGAT4156.14079E-78.1382982
CGTATT2500.00117517138.1057453
ATTCAA20100.08.0654176
TATTCG3701.0774239E-57.903342724
CGGCCT6503.85171E-107.6229781
CGTAAC6301.6998456E-97.5053193
CGGTTC9100.07.419110320
GGCTAA6503.1741365E-97.266290710
TCGAGT14950.07.22920372
TCGACC7803.956302E-117.21069334
ACATTC13850.07.15308434
CTCCGT7955.9117156E-117.08252671
GTAAAA14000.07.07847931
Produced by FastQC (version 0.11.3)