FastQCFastQC Report
Thu 4 Feb 2016
Sample_16677Index__14_20160127_15h_spri_lowallfastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSample_16677Index__14_20160127_15h_spri_lowallfastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2033228
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTGCCAGTGGACACTGTTGGATACATAGTATCTCCTTCAAGTTCCAT721653.5492822251119898No Hit
GTCTTTCACCCTCTCTCACTCTTGTTTTATTTTATCATTATTTTTTTTTT311671.532882687037558No Hit
AAAGTGCCAGTGGACACTGCTGGAAGAGCAAAAATGAAATCATTTCACAA307091.5103569299655524No Hit
AAAGTGCCAGTGGACACTGCTTTAATTGAGGAACAATAACCGTGGATAAC278571.370087368460399No Hit
AAAGTGCCAGTGGACACTGAGAATATCGACGTCCATTTCGACGAAACAAC213091.0480378983567018No Hit
AAAGTGCCAGTGGACACTGCGTGACCGATCATTGAGAAAAATTGATATAT152920.7521045352513344No Hit
AAAGTGCCAGTGGACACTGAGAATATCGACATCCATTTCGACGAAACAAC87290.4293173220120911No Hit
AAAGTGCCAGTGGACACTGTACACAGATGACCTTTTTAGACATGTTATAC86630.42607125221568853No Hit
AAAGTGCCAGTGGACACTGTACACAGATGACCTTTCTAGACAGGTTATAC78260.3849051852522196No Hit
GTCTTTCACCCTCTCTCACTCTCTCTCTCCAACCGAAGCAAGCCATTGCA76270.37511779298730885No Hit
GTCTTTCACCCTCTCTCACTCCCTCTTTCAGACTCTACCCTCTCCCATAT75520.37142907730957864No Hit
AAAGTGCCAGTGGACACTGCGTGACCGATCATTGAGAAAATTTGATATAT73750.3627237083101354No Hit
AAAGTGCCAGTGGACACTGACATGTAAACTGGTTTCCAGCTTCATCAATA72580.35696931185287634No Hit
GTCTTTCACCCTCTCTCACTCTTGTTTTATTTTATCATTATTTTTTTTTC69430.3414767060064095No Hit
GTCTTTCACCCTCTCTCACTCTATATTTCCACCTCTTTATTAATATCTCA51440.252996712616588No Hit
GTCTTTCACCCTCTCTCACTCTCTTGCTCTCCCCTCTCTCTCTCTCTCTC42040.20676480945570294No Hit
GTCTTTCACCCTCTCTCACTCTCTCTCTCTCTCTCACACACACACACACA38990.19176403236626685No Hit
AAAGTGCCAGTGGACACTGCGTGACCGATCATAAGAATGACGGATGGTTC34600.17017274993261947No Hit
GTTTTTCACCCTCTCTCACTCTTGTTTTATTTTATCATTATTTTTTTTTT33710.1657954739950463No Hit
AAAGTGCCAGTGGACACTGTATGGTTTCAGTTATAATAATCATTAACAGG33480.16466426785387572No Hit
AAAGTGCCAGTGGACACTGCGGGACTTGGTGCGAAGCTCTGTGCAGAGTT31720.15600808173013553No Hit
GTCTTTCACCCTCTCTCACTCTCCAACCGAAGCAAGCCATTGCAGAAAAA30530.1501553195214703No Hit
AAAGTGCCAGTGGACACTGGGAATATCGACGTCCATTTCGACGAAACAAC30190.14848310174756593No Hit
GTCTTTCACCCTCTCTCACTCTTCCTTGTGTATTTCCTCTCGCCTTTTAA30010.1475978099849107No Hit
AAAGTGCCAGTGGACACTGTGGACTGGAGAGTGAGAAAATAAAAGATGAA29140.14331889979874368No Hit
GTCTTTCACCCTCTCTCACTCTCTCTCACACACACACACACACATACACG28790.14159749914913625No Hit
AAAGTGCCAGTGGACACTGGAATTGAGAACTCTCCATACCATCACGGCCT27330.13441679929648814No Hit
GTCTTTCACCCTCTCTCACTCTCTTGTTTTATTTTATCATTATTTTTTTT26730.13146582675430402No Hit
AAAGTGCCAGTGGACACTGTGGATGATGGTATAGAAGTCCTCCACCACGG26060.12817057408219837No Hit
GTCTTTCACCCTCTCTCACTCTCTTTCCTATCCCCTCTCTCTCTCTCTCT25650.12615407617837252No Hit
AAAGTGCCAGTGGACACTGCTTTAATTGAGGAACAATAACCGTGAATAGC25280.12433430977735896No Hit
AAAGTGCCAGTGGACACTGTGGACTGGAGAGTCAAAAAAAAAAGAATGAA24350.11976030233697352No Hit
AAAGTGCCAGTGGACACTGTATGGTTTCATTTATAATACTCATTAACAGG24000.11803890168736608No Hit
AAAGTGCCAGTGGACACTGCGTGACCGATCTATGAGCCCTCCAGAGGCGT23980.11794053593595998No Hit
AAAGTGCCAGTGGACACTGACTTGTAAAAACTGACCAGGCTTAACCCAAT22170.10903843543370444No Hit
AAAGTGCCAGTGGACACTGCGGTACTTGGTGCGAAGCTCTGTGCAGAGTT21060.10357913623066375No Hit
AAAGTGCCAGTGGACACTGTTCTATTAGTATAAGCTATAGAAAGGCACTA20410.10038224930996427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTGC968650.091.752062
AGTGCC969400.091.487723
GTGCCA994150.091.2266854
TGCCAG1005300.090.1609655
GCCAGT1005250.089.846686
AAAGTG988850.089.725481
CAGTGG997150.089.4880148
CACCCT653600.089.449957
CCAGTG1006350.089.149127
TTCACC680150.089.081735
AGTGGA1012200.088.504689
ACCCTC665850.087.884928
TCACCC668350.087.802746
GTCTTT643000.087.013381
CTTTCA706600.080.944463
TTTCAC800550.077.825684
CCCTCT824450.071.249199
GTTCCG27400.070.559052
CCGTCC32450.067.180435
TCTTTC835050.067.1525342
Produced by FastQC (version 0.11.3)