FastQCFastQC Report
Thu 4 Feb 2016
Sample_16678Index__15_20160127_expo_spri_lowallfastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSample_16678Index__15_20160127_expo_spri_lowallfastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3568860
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGAGATGAGCTGGCACCTAGAAAAGGAGTGCAAAGTCTCTGGGGT37366110.470038051366542No Hit
TGGAGGCCCAGACCTACTGGCCAGCCTCTCCCAAGTTTCTAACCCAGCTG2208786.189035154082816No Hit
TGGAGGCCCAGACCTACTGAATGCACTAACACCAATCTAAATGTCTTATC1748484.899267553224279No Hit
CATAGAGAGATGAGCTGGCACATAGGTATTGGTTCAGATAACCTGAGATT1694334.747538429638596No Hit
TGGAGGCCCAGACCTACTGCCCAGCCTCTCCCAAGTTTCTAACCCAGCTG1441264.038432440611288No Hit
TGGAGGCCCAGACCTACTCAGCCCCACTCTTGCCAACAGGGATGTCTGGT969242.7158252214993026No Hit
CATAGAGAGATGAGCTGGCACATCGCTGGGGCTGCAGGGGCTGGGTCATG919682.5769573477244836No Hit
CATAGAGAGATGAGCTGGCACAGAAAGCTTGAGTGGATTCCTATCTAAAA903492.5315927214852922No Hit
TGGAGGCCCAGACCTACTGCTGACCCTTAATTCTACGCTCTATTCCAAGG745062.0876694518697847No Hit
TGGAGGCCCAGACCTACTGCCCAGAGTTTACATGACCTGCTGCTGCTGTC484581.3578005301412777No Hit
CATAGAGAGATGAGCTGGCACAAACACTTGCAGCCTAAGGAACACTTGTC471451.3210100704426624No Hit
TGGAGGCCCAGACCTACTTCTAATGTGTATGTACTTCTCTATGGCTGAGC317900.8907606350487269No Hit
CATAGAGAGATGAGCTGGCACACTGATCAGGATGCTATAAGCACTTCATT306220.8580330974036527No Hit
TGGAGGCCCAGACCTACTGCTGCCTTTTCTTCTGCCAGAAGATGGCTATT257370.7211546544274642No Hit
CATAGAGAGATGAGCTGGCACAATTCTGCAGGCCTGTTATCCAGAGAGTC252830.7084335053770672No Hit
TGGAGGCCCAGACCTACTGAATCTGAATCTTGGAATCACGGCGCTCTCTG233220.6534859871219381No Hit
CATAGAGAGATGAGCTGGCACTGCCAGGACTTTGGGGTGGTAGAGGTAGT218440.6120722023279141No Hit
TGGAGGCCCAGACCTACTGAATATGAGCGTGTACTTTGAACAGGTTCCCC177180.4964610547906054No Hit
CATAGAGAGATGAGCTGGCACAACTGGTTGTTATCATGTAGAAGAATGCG171740.4812180920518037No Hit
TGGAGGCCCAGACCTACTGTTCAATAGAAGAAACTGAGGGTGGGAACTGG167580.4695617087809553No Hit
CATAGAGAGATGAGCTGGCACACAATAGCAGACACAGATGTCCTTTAGCA164920.46210834832411474No Hit
TGGAGGCCCAGACCTACTGGTGATGAAGGAAATACTACAAATGACAACAT161350.4521051540267761No Hit
CATAGAGAGATGAGCTGGCACTATCAAGCACACCAGGCAGCCTACAATTT150640.4220955711347601No Hit
TGGAGGCCCAGACCTACTGACATTAGGTTTACATAATTGTTTTAAAGGCT145710.40828163615272106No Hit
CATAGAGAGATGAGCTGGCACACAAGATGCTGAAAATTTACCCAGGATCC144270.40424673425127355No Hit
TGGAGGCCCAGACCTACTGTGGGTGGTGCCATCCCTGGGCAGGTGGTCCT139400.39060092018179476No Hit
CATAGAGAGATGAGCTGGCACACACGCAGCCATAATCTCTGCTACCTGCA136490.38244705592261957No Hit
CATAGAGAGATGAGCTGGCACCTGGGGGCAGGAGCTGATGCAGAGGCCAC131880.3695297658075688No Hit
TGGAGGCCCAGACCTACTGGCCCTGTTCCCGAGGCAGCAACCATGTCATC125370.3512886467947748No Hit
CATAGAGAGATGAGCTGGCACACATCAACTAACCCTTTCTTTGCTTAAAA121630.3408091099118486No Hit
TGGAGGCCCAGACCTACTGTACATTAGCCACAAGATCTCTCTCTTTTCTT112510.315254731202681No Hit
TGGAGGCCCAGACCTACTCTTGGGTGTTGTAGGCTGCCACTTTCCTTCAA93150.26100771674988654No Hit
CATAGAGAGATGAGCTGGCACAGGGAAGGTCATTTCCACATTTCATTTAC89570.2509765023004545No Hit
TTGAGGCCCAGACCTACTGGCCAGCCTCTCCCAAGTTTCTAACCCAGCTG80880.2266269901313024No Hit
TGGAGGCCCAGACCTACTGGAGGGGCCCAGGAGAGACCAGGAGGTCTTTA76470.21427010305811942No Hit
CATAGAGAGATGAGCTGGCACAAATGAACCTCACTGGGGATGGGGAGACT71620.20068032929282742No Hit
CATAGAGAGATGAGCTGGCACACTTGCCGTGTGTGCCCATGGGACACCCT70520.197598112562555No Hit
TGGAGGCCCAGACCTACTGAGCCATCTTGCCTGCCTGTGATGTGGGACTT70140.19653334678300635No Hit
TGGAGGCCCAGACCTACTGAAGGCTCTCACACTTTGGATTCTTCTGCCCT69650.19516035933043044No Hit
CATAGAGAGATGAGCTGGCACACAAAAACTTATGCAAGACACACTTGTTG68560.19210616275225142No Hit
TGGAGGCCCAGACCTACTGTGGCCTTTTATGTTCATCATAAGAAGGTGGA67230.18837948252383113No Hit
TGGAGGCCCAGACCTACTGCCAAGGTGAGAACTATCAATTCCTCCCATGT66970.18765095856940311No Hit
CATAGAGAGATGAGCTGGCACATAAGCCTGCCCTCTGTTATTAGCTATGG65590.1837841775805159No Hit
CATAGAGAGATGAGCTGGCACAGAGCAGACCATGCTTGCTTCTCACATGG65460.1834199156033019No Hit
CAGAGAGAGATGAGCTGGCACCTAGAAAAGGAGTGCAAAGTCTCTGGGGT64420.1805058197855898No Hit
TGGAGGCCCAGACCTACTGACTCTGTAAAGAATCTGGCACAGCAAAAGCA61760.17305245932874921No Hit
TGGAGGCCCAGACCTACTGTGCAGAGTACTAAAGAGGGTAATGCTCATGT56090.15716503309179963No Hit
TGGAGGCCCAGACCTACTGTGGGACAGGCATTCCTGGACAAGTGAGTGGC55880.15657660989783853No Hit
CATAGAGAGATGAGCTGGCACAGCCCCACTGGGCTTCTCTTACGGTTCTG55090.15436301788246107No Hit
CATAGAGAGATGAGCTGGCACAAACCTCGCTTTAGCCTTTTGTGGTACTA53700.15046821674148048No Hit
TGGAGGCCCAGACCTACTGATGCTATGGCTTCTCTTCCCACCATCGTGGG53140.14889908822425088No Hit
TTGAGGCCCAGACCTACTGCCCAGCCTCTCCCAAGTTTCTAACCCAGCTG52810.14797442320516915No Hit
CATAGAGAGATGAGCTGGCACACTTGCTAGGCTCAGGGTTGTCTCTCATC52780.147890362748889No Hit
TGGAGGCCCAGACCTACTGCTGACCCTTAATATCATTGTGGGAGTGGGTT51060.14307089658882668No Hit
CATAGAGAGATGAGCTGGCACTAGGAGGCTGGGTGGGAAGGGGCCATATG48740.13657022130316124No Hit
CATAGAGAGATGAGCTGGCACACTTGCTAGGCTCAGGGTTGTCTCTCGTC46770.13105025134076428No Hit
ATAGAGAGATGAGCTGGCACCTAGAAAAGGAGTGCAAAGTCTCTGGGGTG46650.13071400951564366No Hit
TTGAGGCCCAGACCTACTGAATGCACTAACACCAATCTAAATGTCTTATC45980.12883665932538682No Hit
GAGATGAGCTGGCACCTAGAAAAGGAGTGCAAAGTCTCTGGGGTGAGTGG42950.120346553241091No Hit
TGGAGGCCCAGACCTACTGCTGACCCTTAAACACTCCAGCTTGACACACC42100.11796484031315323No Hit
TGGAGGCCCAGACCTACTGCCATTGCACCTATGCACAATTGGACACGTGT42040.1177967194005929No Hit
CATAGAGAGATGAGCTGGCACACAGGGACTTCATCTAGTCCTAGCATGAT41850.11726433651081858No Hit
TGGAGGCCCAGACCTACTGCAGCCAACCCAGGGTAGCACCCTGGAGGGTG41420.116059469970803No Hit
CATAGAGAGATGAGCTGGCACACAAGCACAAGGCAAAGAGAGCTAACTGG39950.11194050761307531No Hit
TGGAGGCCCAGACCTACTGGCTCTGTTCAGGGTGTAAGGGAGCACTTTCC38600.10815778708046828No Hit
TGGAGGCCCAGACCTACTGAACTAGAATCTTCAACTGACAGAGTCCCACC36140.10126482966549542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGAG1551950.091.66142
CATAGA1555450.091.368761
TGGAGG1447500.090.1447141
CAGACC1542750.090.0144659
CCAGAC1537800.089.8563848
GAGGCC1560450.088.3221363
GGAGGC1515200.087.708992
CCCAGA1601300.086.426587
GAGATG1693700.085.758037
AGATGA1692400.085.69488
GATGAG1714650.085.1894159
GGCCCA1632200.085.127415
GAGAGA1742700.084.030435
AGAGAG1784250.082.430314
AGGCCC1693700.082.0812154
TAGAGA1797850.078.979153
GCCCAG1789700.077.519616
AGAGAT2116050.069.0616
CGTAGA18700.067.8342361
TTGAGG84250.067.2181
Produced by FastQC (version 0.11.3)