FastQCFastQC Report
Tue 21 Aug 2018
2460_S20_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2460_S20_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81746592
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATATCTCGTATGC1073920.13137183749507259RNA PCR Primer, Index 44 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATC1431450.026.407734
CCGACG1511350.025.1903321
CGACGA1842600.021.0301112
GACGAT2219150.017.6063083
CGATCA896800.015.4498045
TCCGAC1344850.014.4448271
CGATCC771250.011.9103415
CGATCT1151900.09.8937265
CGCGAC1206850.09.71114351
ACGTTA273100.09.0317751
TAGGTA993750.08.5681555
GTAGGT1174100.07.55031064
GCGACC1370800.07.30927942
CGATCG1134750.06.58405455
CGACCT1650050.06.561943
GTAAGG1719950.06.49031548
GGTAAG1653000.06.22352557
GGGGGG239760500.05.7407937145-146
AACACG729950.05.65850161
ACGGAC1399300.05.4341921
Produced by FastQC (version 0.11.3)