FastQCFastQC Report
Tue 21 Aug 2018
2460_S20_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2460_S20_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81746592
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2971180.3634622468420457No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1883880.2304536438656672No Hit
ACAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGC1105800.13527169426219016No Hit
CTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATC1040240.12725178806230847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGA3096950.048.1433269
GCACCC3461700.044.6134267
GGCACC3557300.040.7050366
CACCCG4181050.038.9183248
TGGCAC3870700.035.8767785
TTGGCA4756500.028.0542164
CTTGGC6737500.019.1027323
CCTTGG6168600.017.9290732
CGAGAA2036700.015.69854710-14
GCCTTG3685500.015.6161171
CCGAGA2559600.012.083410-14
AACCGA722350.011.7369199
CAACCG641350.010.6140778
GAGAAT3360100.09.62005510-14
AATTCC3853900.09.31201515-19
CCCGAG3245100.09.24100410-14
ACCTTG3136450.08.7538371
CAAACC2268300.08.742822
ACCCGG2022750.08.689419
TTCGGC2099200.08.59900952
Produced by FastQC (version 0.11.3)