FastQCFastQC Report
Tue 21 Aug 2018
2524_S17_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2524_S17_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82005866
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCTCGTATGC1075340.13112964382328454RNA PCR Primer, Index 41 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATC1369950.028.5039774
CCGACG1396350.028.4354111
GACGAT2314950.017.7192523
CGATCA826000.016.8889225
TCCGAC1176750.016.5391771
CGATCC749750.012.8941735
CGATCT1077600.010.9686075
ACGTTA288100.010.51186851
TAGGTA867950.08.9778145
GTAGGT1040750.08.0480294
CGATCG1016000.07.5618625
CGCGAC805550.07.238181
GTAAGG1458600.06.88793668
GGTAAG1431650.06.5691247
AGGTAA1540200.05.81721746
GGGGGG306187950.05.764122145-146
AATGTA2058450.05.66515161
TAAGGG1829500.05.65104689
TGTAGG1881450.05.3826113
AAGGGA4389150.05.2339481
Produced by FastQC (version 0.11.3)