FastQCFastQC Report
Tue 21 Aug 2018
2524_S17_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2524_S17_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82005866
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2049220.2498870019859311No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1945670.23725985650831372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGA3118800.066.830429
CACCCG4101050.056.4297078
GCACCC3941150.056.129227
GGCACC4108600.050.9629866
TGGCAC4368300.045.7818835
TTGGCA5241750.036.5857734
CCTTGG5846900.027.7095762
CTTGGC7067050.026.190583
GCCTTG3937450.021.895771
CGAGAA2575900.017.36980210-14
ACCCGG1684350.016.2484469
AACCGA754000.014.1182569
CCGAGA3080150.013.92062410-14
CAACCG669400.013.0141478
ACCTTG3026100.012.6681021
CCCGAG3565750.011.57887810-14
GAGAAT3917300.011.44576710-14
AATTCC4443650.011.17870515-19
ATTCCA5131800.010.45610815-19
AGAATT4518800.010.31653910-14
Produced by FastQC (version 0.11.3)