FastQCFastQC Report
Tue 21 Aug 2018
2818_S21_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2818_S21_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72412742
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCATTCATCTCGTATGC1084610.1497816503067927RNA PCR Primer, Index 45 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACG1125450.032.6799131
ACGATC1278500.028.1574364
CGACGA1363050.026.8762422
TCCGAC967900.019.0449311
GACGAT2099400.017.800823
CGATCA805950.016.5939245
CGATCC665200.013.4033595
ACGTTA265600.012.0893011
CGATCT1048100.010.5185645
CACCCG1248700.09.8863726
CGATCG892450.07.2924875
CGTTAA465700.07.12987232
CTTGGC2638700.06.11193661
ATTAAC1262000.06.08899451
ACCCGA1146000.05.83179957
GGGGGG188455550.05.8123107145-146
GTACAC1244600.05.78014669
TAATCG718500.05.7475715
TTAACC1234750.05.72101262
TTGGCA3012800.05.54436832
Produced by FastQC (version 0.11.3)