FastQCFastQC Report
Tue 21 Aug 2018
2818_S21_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2818_S21_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72412742
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4650440.6422129409213644No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2027040.2799286346593532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGA2773250.088.983079
CACCCG3562350.076.3118068
GCACCC3968550.065.664117
GGCACC4310550.057.5480426
TGGCAC4450200.053.3468065
TTGGCA5397950.042.2191774
CCTTGG5826800.032.552592
CTTGGC7009450.031.1981533
GCCTTG3946450.025.5530051
ACCCGG1218550.025.04029
CGAGAA2741550.019.33242810-14
AACCGA720500.017.3569729
CCGAGA3150150.016.06787910-14
CAACCG609300.016.0341728
ACCTTG2891450.015.0977561
CCCGAG3384600.014.272367510-14
GAGAAT3996500.013.26693510-14
AATTCC4582850.012.62576815-19
AACCCG627450.012.4539598
ATTCCA5058500.012.2660915-19
Produced by FastQC (version 0.11.3)