FastQCFastQC Report
Tue 21 Aug 2018
2819_S22_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2819_S22_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77949602
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC901880.11570039831633777No Hit
AAGGGAAAGTTGAAAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAA838160.10752588576398375No Hit
ATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAACGG792620.10168364939182113No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATC1630300.026.091394
CCGACG1829400.023.1165871
CGACGA2133400.020.499862
GACGAT2092800.020.4473763
CGATCA890250.018.0632465
TCCGAC1687000.014.0847221
TTAGTC968350.012.8315314
TATTAG1046700.011.9987942
ATTAGT1370000.011.79110053
CGATCC897250.011.4169435
CGCGAC1713300.010.7038011
CGATCT1243450.010.0807085
ACGTTA231350.09.3053231
GCGACC1706500.08.7836112
CGACCT1854000.08.7326263
CACCCG1756750.08.5099746
TAGTCA1595000.08.1882025
ATATTA1769300.07.8284121
TAGGTA1122000.07.4587625
ACGGAC1354150.06.9518741
Produced by FastQC (version 0.11.3)