FastQCFastQC Report
Tue 21 Aug 2018
2819_S22_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2819_S22_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77949602
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2935170.3765471438840701No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1942070.24914431250078736No Hit
CTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATC1755470.22520576820905386No Hit
ACAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGC1438410.18453076899610085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCC4912700.065.4055257
GGCACC4736700.064.11896
ACCCGA5278900.057.7833639
TGGCAC5105150.056.735825
CACCCG6429100.052.325568
TTGGCA5736150.048.3297584
CTTGGC8046200.033.119573
CCTTGG7068250.033.0120472
GCCTTG3979300.031.539361
CGAGAA3196000.020.13052410-14
AACCGA795600.018.3047169
CAACCG695900.017.0583868
CCGAGA3705500.016.67328610-14
ACCTTG3341250.015.9215011
GAGAAT4354450.014.48039510-14
ACCCGG2713800.013.9563059
AATTCC4991650.013.92170515-19
AGAATT4867150.013.31273110-14
ATTCCA5760850.012.87647715-19
CCCGAG4736950.012.62335510-14
Produced by FastQC (version 0.11.3)