FastQCFastQC Report
Tue 21 Aug 2018
2884_S19_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2884_S19_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73548614
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGC936000.12726276527794256RNA PCR Primer, Index 43 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATC1341800.026.5353934
CCGACG1528150.023.446791
CGACGA1770050.020.597042
GACGAT1978150.018.4529573
CGATCA828750.015.8644415
TCCGAC1293150.013.8651371
CGATCC701400.012.42035
ACGTTA250450.09.5278441
CGATCT1122250.09.4009595
CGCGAC1314150.08.3571751
CGATCG1017900.07.21801665
TAGGTA921650.07.15642175
CGACCT1519950.06.6723713
GCGACC1406600.06.6198482
GTAGGT1077700.06.3571324
AACACG694600.05.736221
GGGGGG202687800.05.558227145-146
GTAAGG1626550.05.5218118
CGATAA540250.05.5100936
ACGGAC1298350.05.3507311
Produced by FastQC (version 0.11.3)