FastQCFastQC Report
Tue 21 Aug 2018
2884_S19_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2884_S19_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73548614
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2602170.35380272427703396No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1873840.2547757052226708No Hit
CTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATC1149640.15631021952364732No Hit
ACAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGC1144030.15554745871893658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGA3225650.051.470259
GCACCC3438200.050.9961477
GGCACC3474400.047.766336
TGGCAC3752300.042.2667355
CACCCG4374250.042.003298
TTGGCA4497400.033.9015124
CTTGGC6256400.023.551363
CCTTGG5678750.022.5618762
GCCTTG3360100.020.7178731
CGAGAA2137950.016.7509910-14
AACCGA650300.014.0807769
CCGAGA2628600.013.057157510-14
CAACCG574650.013.0395828
GAGAAT3311350.010.839457510-14
ACCCGG2004650.010.63865959
AATTCC3796000.010.50512815-19
ACCTTG2842050.010.4205541
CAAACC2036650.010.3677762
CCCGAG3323650.010.00661410-14
TTCGGC2107850.09.7856472
Produced by FastQC (version 0.11.3)