FastQCFastQC Report
Tue 21 Aug 2018
3020_S18_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename3020_S18_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70787388
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2825870.39920529346272815No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1569760.22175701694205754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGA3339500.072.924849
GCACCC4005300.064.260037
CACCCG4298300.062.5446668
GGCACC4136950.059.031676
TGGCAC4366800.053.4065445
TTGGCA5132050.043.5919464
CCTTGG5750500.032.811752
CTTGGC6860000.031.3981173
GCCTTG3719400.026.9381261
CGAGAA2721550.019.05747410-14
ACCCGG1663300.018.8008829
AACCGA700050.017.4992039
CAACCG603950.015.8016868
CCGAGA3161050.015.737658510-14
ACCTTG2842000.015.6222291
GAGAAT3853350.013.30542310-14
CCCGAG3611050.013.20078110-14
AATTCC4474300.012.71397215-19
AGAATT4387450.012.0631610-14
ATTCCA5086550.011.97758415-19
Produced by FastQC (version 0.11.3)