FastQCFastQC Report
Tue 21 Aug 2018
3225_S13_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename3225_S13_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14683309
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTATGC4015272.7345811492491237RNA PCR Primer, Index 37 (100% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCGCGTATGC1494261.017658894190676RNA PCR Primer, Index 37 (98% over 50bp)
CCTTGGCACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATA962020.6551792923516082RNA PCR Primer, Index 37 (100% over 40bp)
CCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTA828530.5642665423713414RNA PCR Primer, Index 37 (100% over 47bp)
CAAGCATAAAGATGTCATCATCAACCAAGTGTACCGCCGGAAGCACCAGG485940.33094719998060385No Hit
TTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTATG418070.2847246489193955RNA PCR Primer, Index 37 (100% over 49bp)
CTTGGCACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATAT410990.2799028475120969RNA PCR Primer, Index 37 (100% over 41bp)
CTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTAT350090.23842718286457093RNA PCR Primer, Index 37 (100% over 48bp)
CCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCGCGTA298640.20338739721407484RNA PCR Primer, Index 37 (97% over 47bp)
CTTGGCACCCGGAATTCCGGGTGCCAAGGCTGGAATTCTCGGGTGCCAAG262160.17854286114934992No Hit
CCTTGGCACCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAAT227100.15466540954767077RNA PCR Primer, Index 37 (100% over 39bp)
GCCTTGGCACCCGGAATTCCAGGTGCCAAGGCTGGAATTCTCGGGTGCCA166930.1136869080395979No Hit
TTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCGCGTATG162630.11075841283460015RNA PCR Primer, Index 37 (97% over 49bp)
GGGGGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATC159250.10845647939439264RNA PCR Primer, Index 37 (100% over 42bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAA164650.061.2402574
ACGTTA50850.053.6720621
AGCATA213350.048.5950623
CATAAA234200.042.6488465
CCTTGG1077000.037.8827441
CACCCG386750.035.4287646
TAATCG93000.030.2827325
CTTGGC828750.029.4625281
TTGGCA811850.029.0870932
GGCACC804600.028.9359344
GCACCC539850.028.1653615
TGGCAC867250.027.148473
AAGCAT396450.026.9476132
GCACCT592400.026.5659796
ACCCGG401900.025.1251587
GTTAAT133700.023.1379643
GCCTTG638450.023.1225971
ATAAAG452900.023.117476
CGTTAA124650.022.1292722
TTAATC129850.021.7450164
Produced by FastQC (version 0.11.3)