FastQCFastQC Report
Tue 21 Aug 2018
3225_S13_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename3225_S13_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14683309
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1701611.1588736571572524Illumina RNA PCR Primer (100% over 45bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG745080.5074333040324902No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT659350.44904728218959367No Hit
AGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCC364580.24829553066001678Illumina RNA PCR Primer (100% over 42bp)
GGTGCCAAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT335080.22820469146293934Illumina RNA PCR Primer (100% over 35bp)
AGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT177770.12106944013777822Illumina RNA PCR Primer (100% over 44bp)
CTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATC176080.11991847341767445No Hit
AGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCG159820.10884467527040395Illumina RNA PCR Primer (100% over 43bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGGA151280.10302854758419919Illumina RNA PCR Primer (97% over 45bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTATATCTCGGTGGTCGCCGTA150070.10220448265442074Illumina RNA PCR Primer (97% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCACC1346400.066.697386
ACCCGA1168400.066.404959
GCACCC1354050.066.245127
TGGCAC1320400.065.292055
CACCCG1448250.063.1453748
TTGGCA1372250.060.820534
GCCTTG961800.054.3236241
CCTTGG1503450.050.500382
CTTGGC1793150.045.8933073
GGTGCC769300.033.1949581
TGCCAA775000.033.1293533
AAGGGA483700.032.6570667
GCCAAG801400.032.374854
CAAGGG508900.031.2549976
GTGCCA823000.031.2063272
ACCCGG502300.030.1840679
CCAAGG920900.027.8330655
AGGGAT680500.025.2607941
ACCTTG526150.020.6205941
AACCGA147300.020.1103049
Produced by FastQC (version 0.11.3)