FastQCFastQC Report
Tue 21 Aug 2018
3275_S14_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename3275_S14_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72839126
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTATCTCGTATGC1293320.17755841825998847RNA PCR Primer, Index 38 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACG1417650.025.8721731
ACGATC1406350.025.8206714
CGACGA1687250.022.0452292
GACGAT2133400.017.5375773
CGATCA823650.016.0601045
TCCGAC1253700.015.8152271
TAGTCC700300.014.0858638
CGATCC741000.011.8746925
ACGTTA259050.011.2400631
CACCCG1499300.011.1342446
ACTAGT954350.010.5961676
CTAGTC1060000.09.9323367
CGATCT1133350.09.5728265
CGCGAC961600.08.2037161
AACTAG1417350.08.0150235
CTTGGC2905250.07.7792391
GGCACC2782550.07.53593354
TTGGCA2950600.07.38019562
GCACCC2495300.07.13152365
TGGCAC3151500.06.73704623
Produced by FastQC (version 0.11.3)