FastQCFastQC Report
Tue 21 Aug 2018
3275_S14_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename3275_S14_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72839126
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4902710.6730874283142827No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2125940.29186786233541573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGA3931550.075.089769
GCACCC4575650.069.057767
CACCCG4898000.066.976748
GGCACC4689750.063.8522156
TGGCAC4921150.058.2270285
TTGGCA5700000.048.1856884
CCTTGG6137150.037.6720542
CTTGGC7445250.035.540013
GCCTTG4035050.031.5176161
AACCGA729950.021.2704019
ACCCGG1826800.021.0882289
CAACCG640650.019.8392148
CGAGAA3192900.019.7048210-14
CCGAGA3622950.016.5650910-14
ACCTTG2990900.016.4545121
GAGAAT4301750.014.4439610-14
AATTCC4959450.013.86747815-19
CCCGAG4164400.013.76071410-14
ATTCCA5525050.013.22190415-19
AGAATT4833000.013.21102410-14
Produced by FastQC (version 0.11.3)