FastQCFastQC Report
Tue 21 Aug 2018
3299_S15_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename3299_S15_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63147980
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACATCTCGTATGC1383060.21901888231420863RNA PCR Primer, Index 39 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATC1158400.024.7088184
CCGACG1219300.023.610511
CGACGA1481850.019.8852462
GACGAT1835750.016.060843
CGATCA704950.015.0693775
TCCGAC1073100.014.53906251
CGTTTC498650.014.1367711
ACGTTA219950.012.5411321
CGATCC630600.010.9569135
CGATCT954650.08.9419855
CGCGAC901250.08.663781
CACCCG1211800.07.3816226
GCGACC1061050.06.4442542
CGATCG952150.05.92333565
CGACCT1275200.05.8989313
GCACCG1001450.05.8648539
GGGGGG173646850.05.658368145-146
ATTAAC954250.05.5672171
ACGGAC1073550.05.52633521
TAATCG613300.05.4961845
Produced by FastQC (version 0.11.3)