FastQCFastQC Report
Tue 21 Aug 2018
3299_S15_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename3299_S15_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63147980
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3016050.47761622778749213No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1736910.2750539288826024No Hit
CTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATC735110.11641069120500766No Hit
ACAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGC710880.112573672190306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGA2989950.063.7317929
GCACCC3426950.058.749437
GGCACC3470950.054.859986
CACCCG3861100.054.3695538
TGGCAC3689000.049.2083175
TTGGCA4400450.039.7486234
CCTTGG4919950.029.808732
CTTGGC6030100.027.8606193
GCCTTG3182550.025.0767251
CGAGAA2231700.018.26908910-14
AACCGA608000.016.9684669
CAACCG531200.015.5483798
ACCCGG1624350.014.9620739
CCGAGA2615000.014.88355110-14
ACCTTG2488200.013.210451
GAGAAT3142200.012.86593210-14
AATTCC3626900.012.361443515-19
CCCGAG3181850.011.79983510-14
ATTCCA4202550.011.48454615-19
AGAATT3630850.011.4736610-14
Produced by FastQC (version 0.11.3)